REGULATION OF VAGAL SUBSTANCE P SYNTHESIS IN VITRO

迷走神经 P 物质体外合成的调节

• 批准号: 3398728
• 负责人: DAVID B MACLEAN
• 金额: $ 10.64万
• 依托单位: RHODE ISLAND HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1989-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3398728
• 关键词: adrenocorticotropic hormone; chromatography; corticosterone; cyclic AMP; efferent nerve; electrostimulus; ganglions; guinea pigs; high performance liquid chromatography; hormone regulation /control mechanism; innervation; insulin; medulla oblongata; neural information processing; neural inhibition; neural initiation; neural transmission; neuroendocrine system; neuronal transport; neuropeptides; neurotransmitter biosynthesis; neurotransmitter metabolism; phosphatidylinositols; radioimmunoassay; sensory mechanism; serotonin; substance P; tissue /cell culture; triiodothyronine; vagus nerve

Substance P, a widely distributed neuropeptide, has been identified within cell bodies of the sensory ganglia of the vagus nerve and the fibers emanating bidirectionally from them. Upon vagal sensory stimulation, substance P may be released not only at central terminals within the medulla oblongata but also at peripheral nerve endings where it may importantly influence cardiovascular, respiratory and gastrointestinal function. This project will study the factors, largely unknown, regulating the synthesis and transport of substance P within the sensory vagus nerve. Using explants of nodose ganglion and vagus nerve that have been demonstrated to synthesize and transport substance P in vitro for periods exceeding 24 hours, these studies will test the hypothesis that substance P synthesis is regulated in one of three ways: 1) by hormones or neurotransmitters acting directly on sensory cell bodies, 2) by the frequency or intensity of afferent neural stimulation or 3) by alterations in neurotransmission of the afferent (central) projection of the bipolar sensory fibers. Synthesis and transport of substance P and of de novo synthesized radiolabeled substance P will be measured during the addition to the explant medium of hormones or selected neurotransmitters or following electrical stimulation of explant peripheral and central projections. Changes in synthesis/transport will be correlated with alterations in two major intracellular second messenger systems, cyclic AMP and polyphosphoinositide metabolism. In vivo studies will also be performed to determine whether altered substance P synthesis and transport is coupled to similar changes in its basal and stimulated release at central brainstem and peripheral vagal sensory terminals. The vagus nerve has important functions in cardiovascular diseases such as arrythmia and hypertension, bronchospastic disorders of the lung and a variety of gastrointestinal disorders. Sensory vagus-contained substance P may play important afferent and efferent roles in those conditions. Understanding the regulation of its synthesis and transport will provide a groundwork for the recognition of and more selective therapeutic intervention in the vagal component of those disorders. These studies also will enhance understanding of substance P and neuropeptide regulation in other parts of the nervous system, including the brain.

P物质是一种广泛分布的神经肽，已在

项目成果

Adrenocorticotropin-adrenal regulation of transported substance P in the vagus nerve of the rat.

促肾上腺皮质激素-肾上腺对大鼠迷走神经中 P 物质转运的调节。

• DOI: 10.1210/endo-121-4-1540
• 发表时间: 1987
• 期刊: Endocrinology
• 影响因子: 4.8
• 作者: MacLean,DB

Substance P content in cultured neonatal rat vagal sensory neurons: the effect of nerve growth factor.

培养的新生大鼠迷走神经感觉神经元中 P 物质的含量：神经生长因子的作用。

• DOI: 10.1016/0006-8993(88)90056-x
• 发表时间: 1988
• 期刊: Brain research
• 影响因子: 2.9
• 作者: MacLean,DB;Lewis,SF;Wheeler,FB
• 通讯作者: Wheeler,FB

De novo synthesis and axoplasmic transport of [35S]methionine-substance P in explants of nodose ganglion/vagus nerve.

结状神经节/迷走神经外植体中[35S]甲硫氨酸-P物质的从头合成和轴浆运输。

• DOI: 10.1016/0006-8993(84)90155-0
• 发表时间: 1984
• 期刊: Brain research
• 影响因子: 2.9
• 作者: MacLean,DB;Lewis,SF
• 通讯作者: Lewis,SF

Substance P and somatostatin content and transport in vagus and sciatic nerves of the streptozocin-induced diabetic rat.

链佐星诱导的糖尿病大鼠迷走神经和坐骨神经中 P 物质和生长抑素的含量和转运。

• DOI: 10.2337/diab.36.3.390
• 发表时间: 1987
• 期刊: Diabetes
• 影响因子: 7.7
• 作者: MacLean,DB

Basal and stimulated release of substance P from dissociated cultures of vagal sensory neurons.

迷走感觉神经元分离培养物中 P 物质的基础释放和刺激释放。

• DOI: 10.1016/0006-8993(90)90093-q
• 发表时间: 1990
• 期刊: Brain research
• 影响因子: 2.9
• 作者: MacLean,DB;Wheeler,F;Hayes,L
• 通讯作者: Hayes,L