AGING AND SENILE DEMENTIA OF THE ALZHEIMER TYPE

这是我们的计划项目的目标，调查 神经纤维缠结、神经炎和淀粉样蛋白的发病机制 老年痴呆症中的斑块 为此，我们开发了一个 综合方案旨在确定 淀粉样纤维和成对螺旋丝的多肽 (PHF)通过分离和确定的氨基酸序列， 组成多肽。 此信息将用于 制备淀粉样蛋白和PHF特异性抗体、cDNA探针，并 合成淀粉样蛋白和PHF肽。 这些试剂将用于 为了（1）鉴定负责异常纤维形成的细胞， (2)研究淀粉样蛋白和PHF多肽的合成 以及它们在纤维中的组装，（3）鉴定淀粉样蛋白和PHF 特定表位并建立其结构关系 淀粉样纤维和PHF，以及（4）识别和评估候选者 正常和AD/SDAT CNS，血清中的前体蛋白 和CSF。

It is the goal of our Program Project to investigate the pathogenesis of neurofirbrillary tangles and neuritic and amyloid plaques in Alzheimer's disease. To do this we have developed an integrated program aimed at defining the constituent polypeptides of the amyloid fibers and paired helical filaments (PHF) by isolating and determining the amino acid sequence of the constituent polypeptides. This information will be used to prepare amyloid and PHF specific antibodies, cDNA probes, and synthetic amyloid and PHF peptides. These reagents will be used to (1) identify the cells responsible for abnormal fiber formation, (2) investigate the synthesis of amyloid and PHF polypeptides and their assembly in fibers, (3) identify amyloid and PHF specific epitopes and establish their structural relationship to amyloid fiber and PHF, and (4) identify and evaluate candidate precursor proteins in both the normal and AD/SDAT CNS, serum and CSF.