FREE RADICALS & AGE-INDUCED NEUROCHEMICAL CHANGES IN THE MONKEY

这项研究的目标是确定氧衍生的作用 大脑老化过程中的自由基。这一目标将通过以下方式实现 获得年龄对自由的不同方面的影响的测量 猴脑几个区域的形成和代谢。这些参数 检测指标包括脂质过氧化、超氧化物歧化活性 超氧化物歧化酶、谷胱甘肽过氧化和过氧化氢酶，游离浓度 自由基清除剂，包括谷胱甘肽、抗坏血酸和尿酸，以及 脑部上清液抑制自由基形成的能力。我们的 清道夫测量方法，高效液相色谱-库仑检测使用16 频道探测器系统，将允许我们同时确定 单胺、其前体和代谢物的浓度 组织样本。有关选定的活动的其他信息 神经递质系统将通过胆碱的测量获得。 乙酰转移酶活性和GABA浓度。两者之间的关系 本课程将探讨自由基代谢和神经递质水平。在……里面 此外，本项目中测量的所有变量都将与 行为、生化和形态与年龄相关的变化 同样的猴子。

The goal of this investigation is to determine the role of oxygen-derived free radicals in aging of the brain. This goal will be approached by obtaining measurements of the effect of age on different aspects of free formation and metabolism in several areas of monkey brain. The parameters to be measured include lipid peroxidation, activity of superoxide dismutase, glutathione peroxidation, and catalase, concentrations of free radical scavengers including glutathione, ascorbic acid and uric acid, and the ability of brain supernatant to inhibit free radical formation. Our method of scavenger measurement, HPLC with coulometric detection using a 16 channel detector system, will allow us to determine simultaneously concentrations of monoamines, their precursors and metabolites in the same tissue sample. Additional information on the activity of selected neurotransmitter systems will be obtained by the measurement of choline acetyltransferase activity and GABA concentrations. Relationship between free radical metabolism and neurotransmitter levels will be explored. In addition, all variables measured in this project will be correlated with behavioral, biochemical and morphological age-related changes found in the same monkeys.