EFRI-EPSRC "ENG-EPSRC EFRI ELiS: Developing probiotic interventions to reduce the emergence and persistence of pathogens in built environments"

ENG-EPSRC EFRI ELIS项目：开发益生菌干预措施，以减少建筑环境中病原体的出现和持续时间，是一个国际性的多学科研究项目，旨在解决当代设计和建设健康建筑环境的议程。该项目汇集了微生物学、建筑环境、传染病和抗菌素耐药性(AMR)方面的专业知识。该提案回应了改善建筑环境健康的紧迫性，采用了一种背离现代理解的方法，即健康的环境应该建立在更少的微生物的基础上。城市化、室内生活方式和根深蒂固的抗生素心态正在选择AMR，目前的大流行有可能加剧我们对抗生素方法的过度依赖，这正在推动其他意想不到的、更长期的公共卫生问题。这种方法考虑了对微生物更细微的理解，认识到并不是所有的微生物都是致病的。通过这种方式，未来健康的建筑应该着眼于区分好的和坏的微生物，并在这样做的过程中，找到方法，既可以减少有害微生物的暴露，又可以允许良性环境微生物的存在和作用，对人类健康和建筑物和城市的弹性有利。该提案将为含有枯草杆菌活菌株的建筑开发新型益生菌材料。枯草杆菌是一种土壤来源的细菌，它具有抑制抗药性生物生长的机制。在实验室中，我们将设计这些益生菌材料，用于建筑物，以证明长期生存和防止AMR细菌在这些材料和其他建筑表面定植的能力。在研讨会上，我们将开发新的生物制造方法，使这些活材料能够被制造成一系列1：1的活建筑构件原型。这些原型将包括地板和墙面、家具部件、建筑面板和覆层，将在纽卡斯尔大学的OME、HBBE的真实建筑环境中进行纵向微生物研究，解决如何将这种方法应用于建筑应用的长期问题。

The project 'ENG-EPSRC EFRI ELiS: Developing probiotic interventions to reduce the emergence and persistence of pathogens in built environments' is an international, multidisciplinary research project that addresses contemporary agendas towards designing and buildings healthy built environments. The project brings together expertise in microbiology, the built environment, infectious disease and antimicrobial resistance (AMR).The proposal responds to the urgency for improving the health of our built environments using an approach that departs from the modern understanding that healthy environments should be based on fewer microbes. Urbanisation, indoor lifestyles and ingrained antibiotic mentalities are selecting for AMR and there is a risk that the current pandemic exacerbates our overreliance on antibiotic approaches which are driving other unintended, longer term public health problems. This approach considers a more nuanced understanding of microbes that recognises that not all microbes are pathogenic. In this manner, future healthy buildings should aim to discriminate between good and bad microbes and in doing so, find ways that can reduce exposure to harmful microbes but also permit the presence and agency of benign environmental microbes roles that are beneficial for human health and the resilience of buildings and cites. The proposal will develop novel probiotic materials for buildings that contain living strains of B.subtilis, a soil derived bacteria that exhibits mechanisms which can inhibit the growth of drug resistant organisms. In the laboratory, we will engineer these probiotic materials for application in buildings that can demonstrate long term survival and ability to prevent AMR bacteria colonisation on these materials and on other building surfaces. In the workshop we will develop novel bio-fabrication approaches that will allow for these living materials to be manufactured in to a series of 1:1 living building component prototypes. These prototypes which will include floor and wall surfaces, furniture components and building panels and cladding will undergo a longitudinal microbial study in a real world building environment at OME, HBBE at Newcastle University, addressing longer term questions of how to progress this approach for building application.