LIVER BIOPSY ASSOCIATED WITH CHOLECYSTECTOMY IN A SINGLE PATIENT

肝细胞将从肝活检标本中获得 一次选择性胆囊切除术分析突变型和突变型 正常人肝细胞载脂蛋白B基因表达水平 家族性低脂蛋白血症杂合子个体。这个 截短(突变)apo-B蛋白的相对合成速率和 全长apo-B蛋白也将通过35S代谢测定 培养肝细胞的标记研究。

Hepatocytes will be obtained from a liver biopsy specimen at the time of an elective cholecystectomy to analyze the relative levels of mutant and normal apolipoprotein B mRNA levels in the hepatocytes of a single individual who is heterozygous for familial hypobetalipoproteinemia. The relative rates of synthesis of the truncated (mutant) apo-B protein and the full-length apo-B protein will also be determined by 35S metabolic labeling studies in cultured hepatocytes.