DYnamic control in hybrid plasmonic NAnopores: road to next generation multiplexed single MOlecule detection

在探索开发平台来破译生物分子中的信息的过程中，对能够以尽可能小的浓度识别特定生物分子，并在纳米尺度上控制它们的捕获和运动的技术的需求越来越大。纳米孔测序是推动这一进步的关键技术，而固态纳米孔可以成为主要的技术参与者。在固态纳米孔领域，仍然存在许多挑战，特别是在i)设计和制造具有与生物孔相当大小的纳米孔，ii)单分子易位的动态控制，以及iii)多路读出。功能纳米材料和DNA纳米技术的发展带来了固态和生物纳米结构集成平台的进展，能够解决纳米孔平台纳米级精度的重要性，并有可能超越生物纳米孔的性能。然而，生物和合成纳米材料的整合在技术上是具有挑战性的，并且它们用于完全控制和测量单分子的应用距离实际应用还很远。DYNAMO被设计成一个创新的研究和培训网络，在那里我们将招募10名研究人员，朝着开发下一代单分子技术的雄心勃勃的目标工作，利用混合DNA和金属纳米结构。该网络汇集了一个独特的团队，由8位世界领先的学者和1家高科技创新公司组成，在光谱学，DNA纳米结构，纳米孔技术开发，单分子检测和控制以及生物技术实际应用的前沿。DYNAMO将与来自6个欧盟国家的合作伙伴在物理/化学/生物技术界面建立一个跨部门的培训和研究项目，旨在培养下一代熟练的、联系良好的科学家，他们将成为“明天的单分子科学”的先驱。

In the quest to develop platforms to decipher the information in biomolecules there is an increasingly demand for techniques that are capable of identifying specific biomolecules at the smallest possible concentration, controlling their capturing and movement at the nanoscale. Nanopore sequencing is a key technology to drive this progress, and solid-state nanopores can be a major technological player. In the field of solid-state nanopores, many challenges remain in particular toward i) design and fabricate nanopore with size comparable to biological pores, ii) dynamic control of single molecule translocation, and iii) multiplexed read-out. Progress toward integrated platforms where both solid-state and biological nanostructures are implemented has been brought by the development of functional nanomaterials and DNA nanotechnology, able to address the importance of nanoscale precision in nanopore platforms with the potential to surpass the performance of biological nanopores. Yet, the integration of biological and synthetic nanomaterials is technically challenging, and their use to fully control and measure single molecule is far from real-world applications. DYNAMO is designed as an innovative research and training network, where we will recruit 10 Researchers to work toward the ambitious goal of developing the next-generation single molecule technologies exploiting hybrid DNA and metallic nanostructures. The network brings together a unique team of 8 world-leading academics and 1 high tech innovative company at the forefront of optical spectroscopy, DNA nanostructures, nanopore technology developments, single molecule detection and control, and biotech real-world applications. DYNAMO will establish an intersectoral training and research programme at the physics/chemistry/biotech interface with partners from 6 EU countries, aimed at creating the next generation of skilled well-connected scientists that will pioneer the 'single molecule science of tomorrow'.