CORE --FLOW CYTOMETRIC DATA COLLECTION AND ANALYSIS (DIDAC)

该项目的总体目标是设计、制造和实施 一种新型通用的流式细胞仪数据采集与分析系统。 本系统将采集数据，控制分拣，有计算机辅助使用 用于控制众多仪器参数的界面，并提供新的 数据显示和分析功能。新系统正在设计中。 并使用最先进的电子电路设计和 制造工具、计算机、操作系统和高级显示器 语言。正在开发的系统已被命名为数字 数据采集与控制系统-DIDAC。我们建议发展这一点 模块化系统，满足四台仪器的个性化需求 安装在NFCR中的多参数流式细胞仪，阶段 灵敏的检测流式细胞仪，光学分选仪，和FAST 动力学流式细胞仪。目标是开发以下模块 整合到仪器中的能力：数据采集 硬件、分拣控制硬件、仪器控制和数据 显示/分析软件。DIDAC系统的实施将需要 NFCR数据采集和显示能力不足，由 当代标准超越了当前最先进的水平。这个 结果将是数据采集系统的改进 NFCR研究仪器和合作者的能力，以及 NFCR技术转让的历史证明，这一点 活动也将对流式细胞仪社区产生很大影响 逍遥法外。

The overall goal of this project is to design, fabricate and implement a new general system for flow cytometric data acquisition and analysis. This system will acquire data, control sorting, have a computer based use interface for control of numerous instrument parameters and provide new data display and analysis capabilities. The new system is being designed and constructed using state-of-the-art electronic circuit design and fabrication tools, computers, operating systems, and high level display languages. The system under development has been given the name Digital Data Acquisition and Control system - DiDAC. We propose to develop this modular system to meet the individualized needs of four instruments housed in the NFCR: the multiparameter flow cytometer, the phase sensitive detection flow cytometer, the optical sorter, and the fast kinetics flow cytometer. The aims are to develop the following modular capabilities for integration into the instruments: data acquisition hardware, sort control hardware, instrument control and data display/analysis software. Implementation of the DiDAC system will take the NFCR data acquisition and display capabilities from inadequate by contemporary standards to beyond the current state-of-the-art. The result will be an improvement in the data acquisition systems capabilities for NFCR research instruments and for collaborators and, as the history of technology transfer from the NFCR has demonstrated, this activity will also have a large impact on the flow cytometry community at large.