GENES REGULATING PATTERN FORMATION DURING EMBRYONIC DEVELOPMENT

建立模式形成所需基因的鉴定 在形态发生过程中及其调控的研究与 脊椎动物生物学的许多方面。形态发生中的许多关键过程， 包括对营养刺激的反应，细胞间的相互作用，迁移， 对差异增殖、程序性细胞死亡等进行了概括。 在肿瘤形成过程中以病理性的方式。所以，对 形态发生过程中的活跃过程及其调控最终将 有助于更好地理解肿瘤发生和发展的机制 转移，并可能提供对癌症预防和治疗的洞察 战略。雏鸡肢体发育是一个很有吸引力的研究系统 花纹形成的分子基础是因为 组织/细胞相互作用的水平已经得到了很好的表征，并 与哺乳动物非常相似，而且这个系统很容易服从于 生化和分子分析以及显微外科操作。 这个项目的目的是分离调控细胞形态发生的基因。 小鸡胚胎的肢芽。目前正在开发两种方法：1) 富含潜在调控功能的消减文库的构建 和诱导基因；以及2)相关/新成员的鉴定 与发育有关的保守基因家族 其他系统中的监管流程。同源异型盒的两个新成员 已发现的基因家族在空间上受到限制 肢芽中的表达结构域，表明在调节中的作用 沿前-后(A-P)和近远端的模式形成 (P-D)肢体的轴。其中一个基因也表达在非常 原肠发育过程中的早期胚胎，以及肢芽，并可 调整沿主线的位置同一性的确定 胚胎轴，以及肢体P-D轴。目前正在进行研究，以 用这两种分子阐明这些调控基因的功能 遗传和生化方法。

The identification of genes necessary for establishing pattern formation during morphogenesis and the study of their regulation are relevant to many aspects of vertebrate biology. Many key processes in morphogenesis, including responses to trophic stimuli, cell-cell interactions, migration, differential proliferation, programmed cell death, etc., are recapitulated in a pathologic manner during oncogenesis. So, an understanding of processes active during morphogenesis and their regulation will ultimately contribute to a better understanding of mechanisms of oncogenesis and metastasis, and may provide insight into cancer prevention and treatment strategies. Chick limb development is an attractive system for studying the molecular basis of pattern formation because critical events at the level of tissue/cellular interactions have been well characterized and are very similar to those in mammals, and this system is readily amenable to biochemical and molecular analysis as well as microsurgical manipulation. The aim of this project is to isolate genes that regulate morphogenesis in the chick embryo limb bud. Two approaches are being developed: 1) the generation of subtracted cDNA libraries enriched for potential regulatory and induced genes; and 2) the identification of related/new members of conserved gene families that have been implicated in developmental regulatory processes in other systems. Two novel members of the homeobox gene family have been identified which show spatially restricted expression domains within the limb bud that suggest roles in regulating pattern formation along the anterior-posterior (A-P) and the proximodistal (P-D) axes of the limb. One of these genes is also expressed in the very early embryo during gastrulation, as well as the limb bud, and may regulate the determination of positional identity along the primary embryonic axis, as well as the limb P-D axis. Studies are underway to elucidate the function of these regulatory genes, using both molecular genetic and biochemical approaches.