EVALUATION OF USEFULNESS OF PCR FOR DIAGNOSIS OF PNEUMOCYSTIS CARINII PNEUMONIA

我们开发了一种快速而高效的方法来提取DNA 呼吸道样本和用户友好的EL ISA格式的聚合酶链式反应产物 检测化验。通过将这两项技术结合起来，我们能够 在诊断实验室中对聚合酶链式反应进行前瞻性评估 作为诊断卡氏肺孢子虫肺炎(PCP)的方法。这个 这项研究的结果表明，使用聚合酶链式反应可以提高 PCP的诊断效率，尤其是来自非侵入性标本的诊断 (诱导痰)。聚合酶链式反应提高了诊断率。 特别明显的是，先天可能会出现 制作含有少量微生物的痰标本(用于 接受化学预防的HIV患者实例，免疫抑制非 艾滋病毒患者)。

We developed a rapid, yet efficient procedure for extracting DNA from respiratory specimens, and a user friendly ELISA format PCR product detection assay. By combining these two technologies we were able to perform, in a diagnostic laboratory, a prospective evaluation of PCR as a method for diagnosing Pneumocystis carinii pneumonia (PCP). The results of this study indicate that the use of PCR could improve the efficiency of diagnosis of PCP, especially from non-invasive specimens (induced sputa). The improvement in diagnosis effected by PCR was particularly marked in patients that, a priori, might be expected to produce sputum specimens containing a low number of organisms (for example HIV patients receiving chemoprophylaxis, immunosuppressed non HIV patients).