AQUA-SYNAPSE: The health benefits of deuterium depletion on synaptic function, regional metabolism, and behaviour

该项目的核心思想是建立贫氘水对健康的积极影响的机制基础，并确定哪些方案最有效。饮用水的同位素含量已被证明可以影响癌症的发病机制，改善应激反应，降低对许多疾病的易感性，但目前尚不清楚饮用水中大量同位素减少的积极生物学效应是如何介导的。高浓度的氘会导致线粒体功能障碍，非常高浓度的氘会导致细胞死亡，但91 ppm的氘在小鼠抑郁症模型中具有与西酞普兰相当的抗抑郁作用。使用贫氘水似乎是一种安全的干预措施，几乎没有副作用，但目前尚不清楚在什么浓度下，积极的代谢和行为影响会变得明显。欧洲的公司现在向公众出售不同浓度的DDW，其中大量的健康声明大多缺乏任何可靠的科学基础或安全数据。因此，迫切需要在分子水平上更好地了解DDW对大脑和行为的影响。我们将使用最先进的成像技术来测量健康个体一生中的动物体内区域代谢通量，以及在不同浓度和不同时期给予DDW后受到压力的动物体内区域代谢通量。此外，我们还研究了氘耗尽如何影响神经元在体外和电生理水平上的行为以及它们的代谢。该项目是通过将化学家、细胞生物学家、药理学家、成像专家、精神病学家和DDW生产商组成的联盟聚集在一起，以完全转化的工作包来发现如何利用DDW操纵代谢途径以造福人类健康而成为可能的

Core idea of this project is to establish the mechanistic basis for the positive health effects of deuterium depleted water and determine what regimens are most efficacious. Isotope content of drinking water has been shown affect the pathogenesis of cancers to ameliorate the stress response and reduce susceptibility to many diseases, but it remains unclear how the positive biological effects of heavy isotope reduction in drinking water are mediated. High concentrations of deuterium induce mitochondrial dysfunction, and very high concentrations can induce cell death, but the administration of deuterium at 91 ppm has antidepressant effects that were comparable to that of citalopram in a mouse model of depression. Use of deuterium depleted water appears to represent a safe intervention with few, if any, reported side effects, but it remains unclear at what concentrations the positive metabolic and behavioral effects become apparent. Companies in Europe now sell DDW at different concentrations to the public amidst a plethora of health claims that mostly lack in any sound scientific basis or safety data Thus, there is an urgent need to better understand the impact of DDW on brain and behavior at a molecular level. We will use state-of-the art imaging techniques to measure regional metabolic fluxes in the body of animals in healthy individuals throughout life and those subject to stress following the administration of DDW at different concentrations and for different periods. Also we examine how deuterium depletion affects the behaviors of neurons in vitro and electrophysiological levels and in terms of their metabolism. The project is made possible bybringing together a consortium of chemists, cell biologists, pharmacologists, imaging specialists, psychiatrists, and DDW producers for 'medicinal needs' in fully translational work packages to discover, in an empiric manner, how the manipulation of metabolic pathways with DDW can be employed for the benefit of human health