STUDIES OF GLOMERULAR CELLS DERIVED FROM NORMAL AND TRANSGENIC MICE

肾小球硬化，以肾小球细胞更新增加为特征 和细胞外基质周转增加，是导致 肾功能衰竭的原因有哪些 目前在人类或动物中的研究 肾小球硬化（GS）模型几乎没有关于 细胞和分子异常，这是至关重要的， 这种疾病的发生和进展。部分原因是， 在体内分离和表征肾小球的困难， 肾小球包含三种固有细胞类型和一个群体， 骨髓来源的巨噬细胞。 这是一个特别的问题， 小鼠，我们选择研究的一个物种， 细胞外基质和生长的大量遗传信息 调节因素。 我们通过分离 单个细胞类型并在体外表征它们。的来源 检查的细胞类型包括正常小鼠、非肥胖糖尿病小鼠和 牛生长激素转基因小鼠。我们首先研究了这个问题 体外肾小球系膜细胞的表型调节， 关于这是否发生的问题， 的发生率，以及是否有不同的系膜 来自不同分离株或不同来源的细胞。 我们发现 不同代次的细胞之间存在显著差异， 不同的分离株和不同的小鼠品系之间。

Glomerulosclerosis, characterized by increased glomerular cell turnover and increased turnover of extracellular matrix turnover, is the leading cause of renal failure in the US. Current studies in humans or animal models of glomerulosclerosis (GS) have yielded little information about the cellular and molecular abnormalities that are critical in the initiation and progression of this disease. This is due, in part, to the difficulty in isolating and characterizing glomeruli in vivo, and the fact that, the glomerulus contains three indigenous cell types and a population of bone marrow-derived macrophages. This is particularly a problem with mice, a species we have chosen to study because of the availability of considerable genetic information about extracellular matrix and growth regulatory factors. We have approached this problem by isolating the individual cell types and characterizing them in vitro. The sources of the cells types examined include normal mice, non-obese diabetic mice, and mice transgenic for bovine growth hormone. We first examined the question of the phenotypic modulation of glomerular mesangial cells in vitro, addressing the question of whether this occurred, what was the time frame of the occurrence, and whether there was a difference between mesangial cells from different isolates or different sources. We found that there was a marked difference between cells at different passages, as well as between different isolates, and different mouse strains.