REGULATION OF EXPRESSION AND FUNCTION OF NEUROPEPTIDES DURING DEVELOPMENT

在过去的一年里， 终止了非洲爪蟾中枢神经系统中的神经肽基因。 但 LIM类家族的一个成员的发育表达 使用原位技术在大鼠小脑中表征同源盒基因 与LCMN的V. Gallo博士合作进行杂交。 北方斑点 分析显示，大鼠小脑中rLIM-1的表达处于高峰 出生后第8天水平，与颗粒细胞的高峰期一致 增殖 在rLIM-1中观察到急剧下降，但内部 颗粒细胞表达低水平。 在发育中的大鼠小脑中， 增殖和迁移前细胞表达高水平的rLIM-1， 在有丝分裂后的细胞中下调，与 通过分子层迁移。

In the past year, the project on the developmental expression of neuropeptide genes in the Xenopus CNS was terminated. However, the developmental expression of one member of the LIM class family of homeobox genes was characterized in rat cerebellum using in situ hybridization in collaboration with Dr. V. Gallo in LCMN. Northern blot analysis showed that rat rLIM-1 expression in cerebellum was at its peak level postnatal day 8, coincident with a peak period of granule cell proliferation. A dramatic decrease was observed in rLIM-1 but internal granule cells express low levels. In developing rat cerebellum, proliferative and premigratory cells express high levels of rLIM-1 but down regulates in post-mitotic cells, coincidental with onset of migration through the molecular layer.