MICROBIAL DETOXICATION/DEGRADATION OF HAZARDOUS WASTES

一个跨学科的基础研究和培训计划， 毒理学家、遗传学家、微生物学家、化学家和工程师， 辛辛那提大学在过去的2.5年里一直在进行，旨在 通过开发新方法减少对人类健康的风险 用于检测有害物质和开发先进的 微生物系统生物降解主要类别的有害化合物， 环境保护 本申请的长期研究目标是 扩展我们的发现和成果， 有关的解毒和生物降解的基础研究问题 危险化合物的复杂混合物。 该提案要求进行培训 和行政核心，以及七个项目。 项目。1处理 酵母细胞色素P450解毒系统的开发， 检测基因探针以监测这些细胞的存活和生长 生物膜中的微生物和其他微生物种群。 项目2 检查新的细菌分离株和微生物财团的好氧 偶氮、Chrome染料及相关染料的解毒、降解。 项目3涉及4和5的遗传毒性和微生物降解 元环芳烃和N-杂环化合物， 煤气化废物场的混合物特性。 项目6寻求 为了了解铬诱变的机制，使用遗传靶点 在哺乳动物和酵母细胞中的穿梭载体中，以及在体外DNA 复制系统。 项目第五章研究了 色谱/等离子体质谱法在铬超痕量分析中的应用 物种形成和其他物种和化合物。 项目。第7章检查 生物膜的操作特性与偶氮生物降解的关系 染料，包括模拟底物运输和化学 生物膜中的反应，并检查厌氧/好氧的效率 反应堆排序 项目8研究绿色环境中的生物降解 根际的植物/土壤/微生物群落。 跨学科，协作 几个研究者的影响构成了一个决定性的特征 整个研究计划。 研究合作也是 招聘和培训MS的跨学科计划的基础， 博士研究生和博士后研究员。 这个程序提供 在研究学科方面的严格训练加上在 对人类健康造成危害的问题和办法 有毒废料场

An interdisciplinary basic research and training program involving toxicologists, geneticists, microbiologists, chemists and engineers at the University of Cincinnati has been underway during the past 2.5 yrs, aimed at reduction of risk to human health through the development of new methods for detection of hazardous substances and the development of advanced microbial systems to biodegrade major categories of hazardous compounds in the environment. The long term research objectives of this application are to extend the discoveries and results we have generated in order to address basic research questions related to the detoxication and biodegradation of complex mixtures of hazardous compounds. The proposal calls for a training and administrative core, and seven projects. Proj. 1 deals with the development of cytochrome P450 detoxication systems in yeasts, and with the examination of gene probes for monitoring the survival and growth of such microorganisms in biofilms and other microbial populations. Proj. 2 examines novel bacterial isolates and microbial consortia for the aerobic detoxication and degradation of azo and chrome dyes and related dye stuffs. Proj. 3 involves the genotoxicity and microbial biodegradation of 4 and 5 membered ring aromatic hydrocarbons and N-heterocyclic compounds that are characteristic of mixtures at coal gasification waste sites. Proj. 6 seeks to understand the mechanisms of chromium mutagenesis, using genetic targets in shuttle vectors in mammalian and yeast cells, and in vitro DNA replication systems. Proj. 5 examines novel approaches in chromatography/plasma mass spectroscopy in ultra-trace analyses of chromium speciation and other species and compounds. Proj. 7 examines the operational properties of biofilms in relation to the biodegradation of azo dyes, including the modeling of substrate transport and of chemical reactions in biofilms, and examining the efficiency of anaerobic/aerobic reactor sequencing. Proj 8 studies biodegradation in the green plant/soil/microflora of the rhizosphere. Interdisciplinary, collaborative effects by the several investigators constitutes a defining characteristic of the overall research program. Research collaborations also are the basis of an interdisciplinary program for recruiting and training of MS and PhD graduate students and postdoctoral fellows. This program provides vigorous training in a research discipline plus broad experience in the problems and approaches to reducing the hazards to human health caused by toxic waste sites.