GENERATION OF AUTOANTIBODIES IN HIV-1 VACCINE GROUPS

(1)项目目标： - 为了确定抗HLA II类自身抗体的贡献， 由于与gp 41的同源性，在HIV-1感染的个体中产生， 疾病进展。 - 为了确定未感染艾滋病病毒的志愿者， 基于疫苗开发抗HLA II类自身抗体。 (2)实验方法： - 来自参与MACS研究的HIV-1患者的连续血清 检测是否存在交叉反应性抗gp 41/II类自身抗体 采用ELISA他们的抗体滴度与他们的疾病相关 在CD 4细胞计数和临床症状方面的进展率。 - 来自接种疫苗的志愿者的血清通过DAIDS小组提供。预 筛选免疫和接种后血清中是否存在抗CLAS II自身抗体。然后将滴度与对以下各项的总体响应相关联： 疫苗和所用的疫苗剂量。 (3)主要发现： - 在一组HIV-1感染的血友病患者中， MACS研究中，发现CRab的高滴度与快速 进展（2-4岁）至完全爆发的艾滋病。 我们目前正在继续研究来自MACS研究患者的血清， 确定CRab自身抗体的存在是否可用作预测 在临床恶化之前进行测量。 - 我们还测试了来自不同疫苗组的血清， 含有gp 41/HLAII类同源区的亚单位疫苗， CRAB的存在。一个血清阳性疫苗组显示， 接种后的CRab滴度。

(1) Goals of project: - To determine the contribution of anti-HLA class II autoantibodies, generated in HIV-1 infected individuals due to homology with gp41, to their disease progression. - To determine whether uninfected volunteers, vaccinated with HIV-1 envelope-based vaccine develop anti-HLA class II autoantibodies. (2) Experimental approach: - Sequential Sera from HIV-1 patients participating in the MACS study are tested for the presence of cross-reactive anti-gp41/class II autoantibodies by ELISA. Their antibody titers are then correlated with their disease progression rate in terms of CD4 cell counts and clinical symptoms. - Sera from vaccinated volunteers are provided through the DAIDS group. Pre immune and post-vaccination sera are screened for the presence of anti-clas II autoantibodies. Titers are then correlated with the overall response to the vaccine and the to the vaccine dose used. (3) Major Findings: - In a cohort of HIV-1 infected hemophiliacs, and patients enrolled in the MACS study, high titers of CRab was found to correlate with rapid progression (2-4 yrs) to full blown AIDS. We are currently continuing to study sera from patients in the MACS study t determine if the presence of CRab autoantibodies can be used as a predictiv measurement preceding clinical deterioration. - We also tested sera from various vaccine groups who were immunized with subunit vaccines containing the gp41/HLA class II homologous region, for th presence of CRab. One seropositive vaccine group showed marked elevation in CRab titers following vaccination.