Instrument Development: A lab-scale soft X-ray microscope for biological systems

本仪器开发应用提出了一种基于实验室，激光驱动，水窗x射线显微镜，能够无标签，纳米空间分辨率，以最小的样品损坏的生物样品。该系统利用了强短脉冲激光脉冲产生的高谐波（HHG）产生的x射线辐射的相干性。相干辐射是使用相位重建算法（例如相干衍射成像和平面摄影）的无透镜成像的基础。该项目建立在南安普顿目前开发的29纳米成像系统的基础上，利用最新的可靠光纤激光技术，并与罗莎琳德富兰克林研究所的目标保持一致，将生产一个在空间分辨率、数据采集时间上增加一个数量级以上的系统，并能够以最小的样品损坏对水合样品进行成像。该系统将在多个合作项目中进行测试，并与RFI和Harwell研究中心的复杂成像能力一起进行测试，并将成为一个可靠的设施，以补充中央激光设施的现有能力

This instrument development application proposes the construction of a laboratory based, laser driven, water window x-ray microscope, capable of label free, nanometre spatial resolution, of biological samples with minimal sample damage.The system makes use of the coherent nature of the x-ray radiation generated by high harmonic generation (HHG) from intense short pulse laser pulses. The coherent radiation is the basis of lensless imaging using algorithmic phase reconstruction (e.g. Coherent Diffraction Imaging and Ptychography).The project builds on the current 29 nm imaging systems developed at Southampton and using recent advances in reliable fibre based laser technologies and in line with the Rosalind Franklin Institutes aims, will produce a system with more than an order of magnitude increase in both spatial resolution, data acquisition time, and enable the imaging of hydrated samples with minimal sample damage.The system will be tested with several collaborations and in conjunction with the RFI and Harwell Research Complex imaging capabilities, and will be engineered to become a reliable facility to complement existing capabilities at the Central Laser Facility