STABILITY OF TETANUS AND DIPHTHERIA TOXINS USING IMMUNOELECTROPHORESIS METHODS

标准品和参比品制备液的校准通常 任意分配;由于参考和标准是 中心的发布和研究活动， 这些材料在化学和免疫学稳定性方面 开始. 采用SDS-PAGE、双向电泳和交叉免疫电泳等方法， 等电聚焦和互补免疫印迹技术， 白喉和破伤风毒素及类毒素制剂， 对温度进行了定性评估。 的工作正在进行 在抑制ELISA的开发中获得定量信息 方法. 这些研究的另一个特点是同时 抗毒素制剂稳定性的评价。 初步 定性分析表明，免疫学特性保留， 比原先预期的时间更长;数据收集是 继续。

Expiration dating for Standard and Reference preparations has often been assigned arbitrarily; since references and standards are a critical part of release and research activities of the Center, the characterization of these materials with respect to chemical and immunological stability has begun. Using methods of SDS PAGE, 2-D, and crossed immuno-electrophoresis, Isoelectric Focusing and complementary immunoblotting techniques, Diphtheria and Tetanus toxin and toxoid preparations stored at different temperatures are being evaluated qualitatively. Work is in progress to obtain quantitative information in the development of inhibition ELISA methods. An additional feature of these studies involves the concurrent evaluation of the stability of anti-toxin preparations. Preliminary qualitative analyses indicate that immunological properties are retained for longer periods than originally anticipated; collection of data is continuing.