PCR 诊断肺囊虫感染的有效性评估-猫眼课题宝

PCR 诊断肺囊虫感染的有效性评估

批准号：

3774519

负责人：

C P CARTWRIGHT

金额：

$0.0万

依托单位：

CLINICAL CENTER

依托单位国家：

美国

项目类别：

财政年份：

资助国家：

美国

项目状态：

未结题

起止时间：

至

来源：

https://reporter.nih.gov/project-details/3774519

关键词：

Pneumocystis pneumonia diagnosis design /evaluation diagnostic tests enzyme linked immunosorbent assay polymerase chain reaction rapid diagnosis

中文摘要

翻译

建立一种基于PCR的诊断肺孢子虫肺炎的方法。一种简单而有效的DNA提取和扩增方法，开发，允许在一天内进行该测定临床微生物学实验室一项前瞻性研究是目前正在比较PCR和FA检测肺孢子虫在呼吸道标本中，初步数据表明，本试验可提高诊断效率，特别是对诱导型你好在迄今为止分析的160份标本中：16份为PCR+/FA+; 6份为 PCR+/FA-（均为痰液，随后确认），0例为PCR-/FA+; 138例 PCR-/FA-。目前正在开发ELISA型检测方法用于检测PCR产物。

英文摘要

Development of a PCR-based assay for diagnosis of Pneumocystis pneumonia. A simple, yet efficient, DNA extraction and amplification protocol has been developed, permitting this assay to be carried out in a single day on the Clinical Microbiology Laboratory. A prospective study is currently underway comparing PCR with FA for detection pf Pneumocystis in respiratory specimens, and preliminary data indicates that the PCR assay can improve the efficiency of diagnosis, specially for induced sputa. Of 160 specimens so far analyzed: 16 were PCR+/FA+; 6 were PCR+/FA- (all were sputum, subsequently confirmed), 0 were PCR-/FA+; 138 were PCR-/FA-. Work is currently underway to develop an ELISA-type assay for detection of the PCR product.

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