MOLECULAR GENETIC EVENTS IN LUNG CANCER

A.心钠素A受体mRNA已被检测到 小细胞肺癌细胞株基因的聚合酶链式反应分析 核糖核酸酶保护试验。与碘化ANF的结合研究表明 可饱和结合但小细胞肺癌上的结合部位数目 细胞系，NCI-H82，NCI-H660，NCI-H1284，从低到准确 估计一下。细胞对外源性添加的心钠素有反应 随着细胞内cGMP的增加，与正常受体相似。 因此，细胞表面似乎存在功能性的心钠素受体。 小细胞肺癌细胞。 B.86名不同个体的血清和尿降钙素水平分别为 包括20名不吸烟者，17名吸烟者，以及 49例小细胞肺癌。尿值最高的是 在这三组个体中不同(p小于或等于 0.00001)。16种肿瘤细胞株的核糖核酸酶保护试验(RPA)和RIA 从这些小细胞肺癌患者中发现有5例患者 血清和/或尿液降钙素水平，但无异位降钙素 在他们的肿瘤细胞系中产生。因此，它似乎提升了 肺癌患者降钙素水平的来源 除了肿瘤细胞。

A. The atrial natriuretic factor A receptor mRNA has been detected on small cell lung cancer cells by PCR analysis of cDNA from the cell lines and RNase protection assay. Binding studies with iodinated ANF showed saturable binding but the number of sites on the small cell lung cancer cell lines, NCI-H82, NCI-H660, NCI-H1284, were to low to accurately estimate. The cells respond to exogenously added atrial natriuretic factor with an increase in intracellular cGMP, similar to the normal receptors. Therefore, there appear to be functional ANF receptors on the surface of small cell lung cancer cells. B. The serum and urine calcitonin values of 86 different individuals were studied by radioimmunoassay which included 20 nonsmokers, 17 smokers, and 49 patients with small cell lung cancer. The urine values were the most different in these three groups of individuals (p is less than or equal to 0.00001). RNase protection assays (RPA) and RIAs of 16 tumor cell lines from these patients with small cell lung cancer showed five had elevated serum and/or urine calcitonin values but did not have ectopic calcitonin production in their tumor cell lines. Therefore, it appears that elevated calcitonin levels in patients with lung cancer can come from a source other than the tumor cells.