TRANSITION-METAL MEDIATED FREE RADICAL FORMATION IN VITRO AND IN VIVO

越来越多的证据表明，可能大多数急性和慢性金属 中毒涉及组织损伤中的自由基反应。自.以来 出现在人体组织中的自由基浓度一般接近 对于ESR谱的灵敏度极限，采用了自旋捕捉技术。 对动物模型的操纵也提供了证据，证明 毒性确实是由自由基介导的。通过成功应用这一点 方法，已经获得了自由基形成的ESR证据 体内产生的铁不仅可以在急性发作后产生，也可以在体内发生 长期的铁摄入量。 铬(VI)化合物对人体健康构成严重危害。 关于它们潜在的呼吸道致癌性和它们的 对其他器官的毒性影响。通过应用ESR和自旋陷阱 技术上，胆汁中也有自由基加合物的存在。 对急性铬(VI)中毒大鼠的影响。还需要额外的测量。 Out提供更多关于 检测到的铬(VI)中毒产生自由基。我们没有 能够显示胆汁或不同组织(脑、 肝、肾)大鼠铅中毒或氨基酮戊酸中毒 酸。我们还发现，铅和铁或铝缺乏毒性协同效应 和铁在这些急性金属-金属中毒之后。

Increasing evidence indicates that probably most acute and chronic metal poisoning involve free radical reactions in tissue injury. Since the concentration of radicals occurring in body tissues is generally near the sensitivity limit of ESR spectroscopy, the spin trapping technique is used. The manipulation of an animal model also provides evidence that the toxicity is indeed free radical mediated. By successfully applying this approach, ESR evidence has been obtained for free radical formation generated by iron may occur in vivo not only after acute but also after chronic iron intake. Chromium (VI) compounds pose a serious occupational health hazard in terms of both their potential respiratory tract carcinogenicity and their toxic effect on other organs. By applying ESR and a spin trapping technique, the existence of a radical adduct has also been shown in bile of rats after acute Cr(VI) poisoning. Additional measurements are carried out to provide more data about the exact mechanism by which the detected free radicals from Cr(VI) intoxication are formed. We were not able to show free radical formation in bile or in different tissues (brain, liver, kidneys) after poisoning of rats with lead or with aminolevulinic acid. We also found lack of toxic synergistic effects of Pb and Fe or Al and Fe after these acute metal-metal poisonings.