MICROBIAL DETOXICATION/DEGRADATION OF HAZARDOUS WASTES

跨学科的基础研究和培训计划，涉及 毒物学家、遗传学家、微生物学家、化学家和工程师 辛辛那提大学在过去的两年半里一直在进行，目的是 通过开发新的方法减少对人类健康的风险 用于检测危险物质和开发先进的 微生物系统可生物降解主要类别的危险化合物 环境。这项应用的长期研究目标是 以扩展我们已产生的发现和结果，以便解决 与脱毒和生物降解有关的基础研究问题 危险化合物的复杂混合物。该提案要求进行一次培训 和行政核心，以及七个项目。项目。%1与 酵母细胞色素P450解毒系统的研究进展 检测用于监测这类动物生存和生长的基因探针 生物膜和其他微生物种群中的微生物。项目。2. 检查好氧细菌的新菌株和微生物组合 偶氮染料、铬染料及相关染料的解毒和降解。 项目。3涉及4和5的遗传毒性和微生物生物降解性 成员环芳烃和N-杂环化合物 煤气化废弃场混合物的特性。项目。6次寻人 利用遗传靶点了解铬的诱变机制 在哺乳动物和酵母细胞的穿梭载体中，以及在体外DNA中 复制系统。项目。5检查新方法在 色谱/等离子体质谱在铬的超痕量分析中的应用 物种形成和其他物种和化合物。项目。7考查 生物膜的运行特性与偶氮染料的生物降解 染料，包括底物运输和化学物质的模拟 生物膜中的反应，以及厌氧/好氧效率的检测 反应堆排序。项目8研究绿色环境中的生物降解 植物/土壤/根际微生物区系。跨学科、协作性 几名调查员的影响构成了一个决定性的特征 整个研究计划的一部分。研究合作也是 跨学科计划的基础，以招募和培训硕士和 博士生和博士后研究员。该计划提供 在研究学科方面的积极培训，以及在 减少空气污染对人类健康危害的问题和途径 有毒废物场地。

An interdisciplinary basic research and training program involving toxicologists, geneticists, microbiologists, chemists and engineers at the University of Cincinnati has been underway during the past 2.5 yrs, aimed at reduction of risk to human health through the development of new methods for detection of hazardous substances and the development of advanced microbial systems to biodegrade major categories of hazardous compounds in the environment. The long term research objectives of this application are to extend the discoveries and results we have generated in order to address basic research questions related to the detoxication and biodegradation of complex mixtures of hazardous compounds. The proposal calls for a training and administrative core, and seven projects. Proj. 1 deals with the development of cytochrome P450 detoxication systems in yeasts, and with the examination of gene probes for monitoring the survival and growth of such microorganisms in biofilms and other microbial populations. Proj. 2 examines novel bacterial isolates and microbial consortia for the aerobic detoxication and degradation of azo and chrome dyes and related dye stuffs. Proj. 3 involves the genotoxicity and microbial biodegradation of 4 and 5 membered ring aromatic hydrocarbons and N-heterocyclic compounds that are characteristic of mixtures at coal gasification waste sites. Proj. 6 seeks to understand the mechanisms of chromium mutagenesis, using genetic targets in shuttle vectors in mammalian and yeast cells, and in vitro DNA replication systems. Proj. 5 examines novel approaches in chromatography/plasma mass spectroscopy in ultra-trace analyses of chromium speciation and other species and compounds. Proj. 7 examines the operational properties of biofilms in relation to the biodegradation of azo dyes, including the modeling of substrate transport and of chemical reactions in biofilms, and examining the efficiency of anaerobic/aerobic reactor sequencing. Proj 8 studies biodegradation in the green plant/soil/microflora of the rhizosphere. Interdisciplinary, collaborative effects by the several investigators constitutes a defining characteristic of the overall research program. Research collaborations also are the basis of an interdisciplinary program for recruiting and training of MS and PhD graduate students and postdoctoral fellows. This program provides vigorous training in a research discipline plus broad experience in the problems and approaches to reducing the hazards to human health caused by toxic waste sites.