ADVERSE ALLERGIC REACTIONS TO VACCINES

以小鼠为靶标的酶联免疫吸附试验 作为捕获和二次抗体的抗人IgE单克隆已被 检测人血清样本中的总IgE。试剂 测试提供了准确的、可重复的读数，使用的是内部 已知IgE含量的标准人血清池。从乙脑病毒获得的血清 参加美国海军免疫计划和JEV的反应堆 在丹麦免疫的反应堆已经接受了总IgE含量的检查。 鼠疫疫苗免疫前后的血清 还对个人进行了总IgE检查。试剂盒的研制 疫苗和疫苗组件的特异性IgE的检测已经被 已启动。检测到鼠疫疫苗特异性IgE已被证实 在有限的测试中。

An enzyme linked immunosorbant assay (ELISA) incorporating mouse anti-human IgE monoclonals as capture and secondary antibodies has been tested for measurement of total IgE in human serum samples. Reagents tested have provided accurate, reproducible readings using an in-house standard human serum pool with known IgE content. Sera obtained from JEV reactors participating in the U.S. Navy immunization program and from JEV reactors immunized in Denmark have been examined for total IgE content. Pre and post-immunization sera obtained from plague vaccine immunized individuals have also been examined for total IgE. Assay development for detection of specific IgE to vaccines and vaccine components has been initiated. Detection of plague vaccine specific IgE has been indicated in limited assays.