COOPERATING ACTIVITY OF HPV-16 AND HSV-2 OF HCMV DNA IN TRANSFORMING HUMAN CELLS

人乳头瘤病毒16型(HPV-16)、单纯疱疹病毒2型 单纯疱疹病毒(HSV-2)和人类巨细胞病毒与宫颈癌有关。 我们检测了HSV-2和HPV-16DNA序列是否可以早期转化 传代人类细胞。牙周成纤维细胞来源于正常牙周组织 个体(N-16)和从增生性牙龈组织 苯妥英钠治疗癫痫患者(R-30)的基因转导 HPV-16DNA单独或与HSV-2DNA联合应用。HPV-16DNA 有效地使R-30细胞永生，但不能使N-16细胞永生。R-30细胞联合 HPV-16和HSV-2DNA的非整倍体增加并生长 在软琼脂中比单独转染人乳头瘤病毒16型DNA的R-30细胞好。两者都有 用Southern印迹和聚合酶链式反应检测HSV-2和HPV-16DNA序列 转化细胞中的分析。我们已经发表了一篇关于这些的论文 数据。我们将检测HPV和HPV的E6和E7基因的表达 这些转化子中细胞癌基因的激活。我们也会 喜欢对人巨细胞病毒DNA序列进行类似的研究。这些数据 提示HPV-16和HSV-2 DNA序列可以多步协同作用 转型的过程。

Human papilloma virus type 16 (HPV-16), herpes simplex virus type 2 (HSV-2) and human cytomegalovirus have been linked to cervical cancer. We examined whether HSV-2 and HPV-16 DNA sequences can transform early passage human cells. Gingival fibroblasts derived from a normal individual (N-16) and from a hyperplastic gingival tissue of an epileptic patient (R-30) treated with phenytoin were transfected with HPV-16 DNA alone or in combination with HSV-2 DNA. HPV-16 DNA efficiently immortalized R-30 cells but not N-16 cells. R-30 cells co- transfected with HPV-16 and HSV-2 DNAs were more aneuploid and grew better in soft agar than R-30 transfected with HPV-16 DNA alone. Both HSV-2 and HPV-16 DNA sequences were detected by Southern blot and PCR analysis in transformed cells. We have published a paper with these data. We will examine the expression of E6 and E7 genes of HPV and activation of cellular oncogenes in these transformants. We would also like to pursue similar studies with HCMV DNA sequences. These data suggest that HPV-16 and HSV-2 DNA sequences can cooperate in multistep process of transformation.