ENDOTOXIN BINDING PROTEIN OF LIMULUS AMEBOCYTE

鲎对入侵微生物的防御机制 一直是我们实验室的主要兴趣之一。 在当前 研究中，我们已经分离出许多蛋白质和肽， 抗菌和/或具有凝集特性。 净化 使用亲和层析的方法， 细菌如脂多糖，导致分离， 表征具有结合能力的12 kD蛋白质 鲎血细胞和革兰氏阳性和革兰氏阴性细菌。 我们一直在研究这种相互作用的性质和特异性， 探索这种和其他成分的凝集素性质， 鲎血淋巴 其他凝集素的部分序列数据已被 获得和这些蛋白质的充分表征是目的， 我们目前的调查。

The defense mechanism of Limulus Polyphemus against invading microorganisms has been one of the main interests of our laboratory. In the current studies we have isolated a number of proteins and peptides with antibacterial and/or with agglutinating properties. The purification procedures using affinity chromatography with surface components of bacteria such as lipopolysaccharide, resulted in the isolation and characterization of a 12 kD protein which has the ability to bind to Limulus blood cells and to both gram positive and gram negative bacteria. We have been studying the nature and specificity of such interactions and exploring the lectin properties of this and other components isolated from Limulus hemolymph. Partial sequence data of other agglutinins has been obtained and the full characterization of these proteins is the object of our present investigations.