LASER SCANNING CONFOCAL MICROSCOPY FACILITY

在过去的三年里，激光扫描共聚焦显微镜 在细胞血液学实验室建立了一个实验室。 共聚焦显微镜是一种最先进的技术， 通过排除离焦来提高显微图像的对比度和分辨率 信息. 获得样品的薄光学切片，每个图像 存储在计算机上，并且图像系列被重建为 立体或三维图像。 该设施已被用于研究 概述如下。 1. 趋化因子刺激中性粒细胞的细胞骨架变化。 丝状肌动蛋白的特异性荧光探针用于绘制 中性粒细胞活化过程中肌动蛋白丝的位置 化学引诱物 肌动蛋白丝定位的时程研究 荧光信号的定量图像分析 进行测定肌动蛋白丝组装的模式， 中性粒细胞刺激时的形态学变化。 2. 白细胞迁移过程中的膜抗原定位。 荧光 嗜中性粒细胞质膜抗原的单克隆抗体用于 检测中性粒细胞上抗原的位置， 体外通过聚碳酸酯膜的化学引诱剂。 结果 使用该技术的研究（J.Immunol.146：949-957， 1991年）。

During the last three years, a laser scanning confocal microscopy facility has been established in the Laboratory of Cellular Hematology. Confocal microscopy is a state-of-the art technology that enhances the contrast and resolution of microscopic images by rejecting out-of-focus information. Thin optical sections of specimen are obtained, each image is stored on a computer, and the image series is reconstructed as a stereo or 3-dimension image. The facility has been used for the studies outlined below. 1. Cytoskeletal changes in chemoattractant-stimulated neutrophils. Specific fluorescent probes for filamentous actin are used to map the location of actin filaments during the activation of neutrophils with chemoattractants. Time course studies of the actin filament location and quantitative image analysis of the fluorescence signals are performed to determine the pattern of actin filament assembly and morphologic change during neutrophil stimulation. 2. Membrane antigen location during leukocyte migration. Fluorescent monoclonal antibodies to neutrophil plasma membrane antigens are used to detect the location of the antigens on neutrophils that have migrated in vitro through polycarbonate membranes to chemoattractants. Results using this technique were published this year (J. Immunol. 146: 949-957, 1991).