FLOW CYTOMETRY FACILITY

流式细胞术已经成为一项有价值的研究和临床应用 用于评估白细胞表型表面标志物的诊断工具 还有血小板。在过去的六年里，一家流式细胞仪设施已经 建立在我们的实验室，已经在几个基本的 和临床免疫学合作研究。举例 今年进行的代表性项目概述如下。 1.血小板抗原表达。与以下机构进行的合作研究 Kristina Prodouz和Elaine Sloand评估了 尿毒症患者糖蛋白lb的变化。研究的结果是 今年出版(BR.J.血醇。77：375-381,1991)。 2.淋巴细胞表型分析。执行的一项合作研究 巴兹尔·戈尔丁分析了小鼠淋巴细胞亚群的变化 体内用抗L3T4抗体处理。研究的结果是 发表于1991年的《艾滋病研究与人类逆转录病毒》。7：471- 481)。 3.人CD45在趋化过程中的表达特征在一个 与密歇根大学肿瘤学系Keith Skubitz合作 明尼苏达州，我们检测了中性粒细胞CD45在迁徙中的表达 和非迁徙的人类中性粒细胞群体。研究结果 是今年出版的(J.免疫。146：949-957,1991)。

Flow cytometry has emerged as a valuable research and clinical diagnostic tool for evaluating phenotypic surface markers on leukocytes and platelets. During the last six years, a flow cytometry facility has been established in our laboratory which has been used in several basic and clinical immunologic collaborative studies. Examples of representative projects conducted this year are outlined below. 1. Platelet antigen expression. A collaborative study undertaken with Kristina Prodouz and Elaine Sloand evaluated the surface expression of glycoprotein lb in patients with uremia. Results of the study were published this year (Br. J. Hematol. 77: 375-381, 1991). 2. Lymphocyte phenotype analysis. A collaborative study performed by Basil Golding analyzed the lymphocyte subset changes in mice that were treated with anti-L3T4 antibody in vivo. Results of the study were published this year (1991 AIDS Research and Human Retroviruses. 7: 471- 481). 3. Characterization of human CD45 expression during chemotaxis. In a collaboration with Keith Skubitz, Oncology Dept., University of Minnesota, we have examined neutrophil CD45 expression on the migrating and nonmigrating populations of human neutrophils. Results of the study were published this year (J. Immunol. 146: 949-957, 1991).