PREPARATION AND CHARACTERIZATION OF POLYCLONAL ANTI-RPRESSS OF HBSAG

用重组PreS1多次注射免疫两只兔 利用商业Ribi佐剂系统的多肽(RpreS1)。血清 通过免疫印迹和免疫印迹筛选rpreS1特异性抗体 放射免疫分析；几乎没有检测到特异性抗体。二 然后用相同的免疫原免疫另外几只兔子 弗洛因德的佐剂，尽管观察到了良好的反应， 抗血清不是PreS1的特异性抗体，提示rpreS1 免疫基因并不纯净。在这些血清中观察到的抗PreS1效价 低于通过免疫获得的先前制剂的含量 将合成的前S1肽(残基21-47)偶联到锁孔上 帽状血蓝蛋白，也低于商品血蓝蛋白 现有的抗PreS1的单抗。因为rpreS1的制备是 最近发现含有两种多肽，可在10%的Tricine凝胶中分离， 我们现在已经用从这些分离出来的材料免疫了两只兔子 乐队。人们希望这将产生特异性抗体。 PreS1。

Two rabbits were immunized with multiple injections of recombinant preS1 peptide (rpreS1) utilizing the commercial Ribi Adjuvant System. Sera were screened for specific anti-rpreS1 by immune blotting and by radioimmunoassay; very little specific antibody was detected. Two additional rabbits were then immunized with the same immunogen in Freund's Adjuvant, and although a good response was observed, the antisera were not specific for preS1, suggesting that the rpreS1 immunogen was not pure. The anti-preS1 titers observed in these sera were lower than those of a previous preparation obtained by immunization with a synthetic preS1 peptide (residues 21-47) conjugated to keyhole limpet hemocyanin, and they were also lower than that of commercially available monoclonal anti-preS1. Because the rpreS1 preparation was recently found to contain two peptides, separable on 10% Tricine gels, we have now immunized two rabbits with the material from these separated bands. It is hoped that this will yield specific antibodies against preS1.