STUDIES ON THE NATURE AND FUNCTION OF THE PHOSPHOPROTEIN, PROSOLIN

我们对Prosolin进行了广泛的表征，它是一种主要的胞浆蛋白 HL-60早幼粒细胞白血病细胞的快速增殖 促肿瘤细胞处理后的磷酸化反应 佛波酯像TPA一样是导致戒断的早期步骤 细胞生长和单核细胞分化的开始。普罗索林一直是 人外周血淋巴细胞(PBL)增殖中的鉴定 二维分离蛋白的胰酶解肽图谱 电泳胶。虽然几乎不在静息PBL中，但Prosolin 有丝分裂原诱导的表达是晚期事件，在发病过程中 S--阶段。它的合成水平与普遍存在的DNA水平相关 综合。普罗索林在外周血淋巴细胞增殖过程中的快速磷酸化 对TPA或A23187治疗的反应，以及对DNA的抑制 综合。Prosolin的磷酸化可能是其部分机制之一 哪些自然产生的生长抑制因子终止正常 淋巴细胞增殖。T细胞白血病细胞系表现出明显的 A23187和A23187对前列腺素磷酸化的抑制作用 其他特工。我们正在克隆和测序前列腺素的cDNA。部分 氨基酸序列数据通过分析胰蛋白酶多肽获得 从双向电泳胶中分离出的Prosolin。堕落 合成了寡核苷酸探针，并用于探测淋巴母细胞 C DNA文库。

We have extensively characterized prosolin, a major cytosolic protein of proliferating HL-60 promyelocytic leukemia cells that undergoes very rapid phosphorylation in response to treatment of the cells with tumor-promoting phorbol esters like TPA as an early step in a pathway leading to cessation of cell growth and onset of monocytoid differentiation. Prosolin has been identified in proliferating human peripheral blood lymphocytes (PBL) by tryptic peptide mapping of proteins isolated from two-dimensional electrophoretic gels. While virtually absent from resting PBL, prosolin expression is induced by mitogens as a late event, during the onset of S-phase. Its level of synthesis correlates with the prevalent level of DNA synthesis. Prosolin undergoes rapid phosphorylation in proliferating PBL in response to TPA or A23187 treatment, together with an inhibition of DNA synthesis. Phosphorylation of prosolin may be part of the mechanism by which naturally occurring growth inhibitory factors terminate normal lymphocyte proliferation. T-cell leukemia cell lines show a marked reduction in the phosphorylation of prosolin in response to A23187 and other agents. We are cloning and sequencing cDNAs to prosolin. Partial amino acid sequence data were obtained by analysis of tryptic peptides of prosolin isolated from two-dimensional electrophoretic gels. Degenerate oligonucleotide probes were synthesized and used to probe a lymphoblast cDNA library.