HEMOGLOBIN-OXYGEN EQUILIBRIUM STUDIES

我们最近收购了Hemox分析仪， 记录全血和分离的氧平衡曲线 血红蛋白该系统将用于确定： 1)氧平衡曲线，以及确定了一些 重要的和相关的变量，如血红蛋白协同性，Boher 影响，温度影响等，对于一些化学改性 血红蛋白制剂。 2)成人镰状细胞病患者的全血氧平衡曲线 正在接受重组人胰岛素联合治疗的患者 促红细胞生成素和羟基脲。胎儿血红蛋白增加的影响 氧结合的合成（EPO/HU给药的结果） 将确定红细胞内血红蛋白S的特征。 这个项目的具体部分将在我们的实验室进行， 与G博士合作细胞实验室的罗杰斯和谢克特 生物学，NIDDK，10号楼。

We have recently acquired The Hemox Analyzer which enables automatic recording of oxygen equilibrium curves of whole blood and isolated hemoglobins. This system will be utilized in determining: 1) Oxygen equilibrium curves as well as the determination of a number of important and relevant variables such as hemoglobin cooperativity, Boher effect, temperature effect, etc. for a number of chemically modified hemoglobin preparations. 2) Whole blood oxygen equilibrium curves in samples from adult sickle cell patients undergoing treatment with a combination therapy of recombinant erythropoietin and hydroxyurea. The effect of increased fetal hemoglobin synthesis ( a result of EPO/HU administration) on oxygen binding characteristics of the intraerythrocytic hemoglobin S will be determined. This specific part of the project will be carried out here at our lab in collaboration with Drs. G. Rodgers and A Schechter from the Lab of Cell Biology, NIDDK, Bldg. 10.