INTERLEUKIN-1 INDUCES INTERLEUKIN-6 PRODUCTION IN PERIPHERAL BLOOD MONOCYTES

白细胞介素-6（IL-6）是单核细胞产生的多功能细胞因子， 成纤维细胞，内皮细胞和角质形成细胞，是由各种诱导 刺激信号，包括脂多糖（LPS），聚，（I），聚 (C)、IL-1、肿瘤坏死因子（TNF）和血小板衍生生长因子。 这些信号中的一些仅在一种细胞类型中有效地诱导IL-6，并且 这种诱导的选择性可以解释生物效应的选择性。 在目前的研究中，我们表明，IL-1 β，。 在成纤维细胞、内皮细胞中有效诱导IL-6， 角质形成细胞，但不是单核细胞，也是一个有效的诱导IL-6， 外周血单核细胞高水平的IL-6活性可能是 中和的特异性抗体IL-6的检测上清液中， IL-1刺激的单核细胞。最大诱导所需的IL-1浓度 10 ng/ml。十二烷基硫酸钠-聚丙烯酰胺凝胶法 在还原条件下电泳，IL-6种类的相对 分子量为19至26 Kd的蛋白质可以特异性地从 IL-1诱导的单核细胞的上清液。据报道， IL-6在多种细胞类型中产生的特征，以及单核细胞衍生的 由IL-1或LPS诱导的IL 6显示相似的大小异质性。的 使用的高度纯化的重组IL-1 β制剂含有很少的，如果 任何，LPS。此外，由IL-1 β诱导的单核细胞产生IL-6， 被抗IL-1 β抗体特异性中和，表明 IL-1不是IL-1制剂中的污染物， 负责IL 6诱导。一些生物学活动已经 IL-1和IL-6。IL-1诱导IL-6的发现， 单核细胞可能有助于确定每个细胞的生物活性谱， 这些相互作用的细胞因子。

Interleukin-6 (IL-6), a multifunctional cytokine produced in monocytes, fibroblasts, endothelial cells, and keratinocytes, is induced by a variety of stimulating signals, including lipopolysaccharide (LPS), poly,(I), poly (C), IL-1, tumor necrosis factor (TNF), and platelet-derived growth factor. Some of these signals induce IL-.6 effectively only in one cell type, and this selectivity of induction may explain selectivity of biologic effects. In the present study, we show that IL-1 beta,.previously known to be a potent inducer of IL-6 in fibroblasts, endothelial cells, and keratinocytes, but not in monocytes, is also a potent inducer of IL-6 in peripheral blood monocytes. High level IL-6 activity that could be neutralized by specific antibodies to IL-6 was detected in supernatants of IL-1-stimulated monocytes. Maximal induction required IL-1 concentrations of 10 ng/ml. As judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions, IL-6 species of relative molecular mass of 19 to 26 Kd could be specifically immunoprecipitated from supernatants of IL-1-induced monocytes. Size heterogeneity is a reported feature of IL-6 produced in a variety of cell types, and monocyte-derived IL6 induced by either IL-1 or LPS displayed similar size heterogeneity. The highly purified recombinant IL-1 beta preparation used contained little, if any, LPS. In addition, monocyte production of IL-6, induced by IL-1 beta, was specifically neutralized by anti-IL-1 beta antibodies, demonstrating that IL-1, rather than a contaminant in the IL-1 preparation, was responsible for IL6 induction. A number of biologic activities have been ascribed both to IL-1 and IL-6. The finding that IL-1 induced IL-6 in monocytes may help in defining the spectrum of biologic activities of each of these interactive cytokines.