BIOLOGIC PROPERTIES OF NUCLEAR ONCOGENES AND ATTEMPTS TO BLOCK THEIR EFFECTS

分子生物学的最新发展导致了 特定的遗传病变导致激活或失活的 各种肿瘤系统中的关键靶基因。这些基因被称为癌基因， 参与调节细胞生长的各个方面。现在 了解这些基因运作的精确机制至关重要 因此最终可以衍生出分子试剂来改变或抑制它们的 方面的影响. 我们选择探索2的生物学和生化功能， 显性（L-myc和c-jun）。 转录和翻译产物 L-myc已经被鉴定，现在正与生物学相关。 功能协调发展的最终，该基因的截短片段将被测试， 电位转换抑制功能 同样，我们最近描述了c-jun在细胞中的转化功能。 哺乳动物细胞，现在正在通过缺失突变来绘制这一功能。 该功能与c-jun的其他已知活动的相关性，例如 将进行反式激活。能够抑制AP-1的c-jun突变体 将表征链活化和细胞转化。

Recent developments in molecular biology has led to the identification of specific genetic lesions resulting in either activation or inactivation of key target genes in various tumor systems. These genes, called oncogenes, are involved in various aspects in the regulation of cell growth. It is now critical to understand the precise mechanism by which these genes function so molecular agents ultimately can be derived to alter or repress their effects. We have chosen to explore the biologic and biochemical functions of 2 dominant (L-myc and c-jun). Transcriptional and translational products of L-myc have been characterized and are now being correlated with biologic functions. Ultimately, truncated fragments of this gene will be tested for potential transformation suppression function. Likewise, we have recently described the transforming function of c-jun in mammalian cells and are now mapping this function by deletion mutation. Correlation of this function with other known activities of c-jun, such as transactivation will be done. Mutants of c-jun capable of inhibiting AP-1 strans activation and cellular transformation will be characterized.