DNA SYNTHESIS FACILITY

合成具有特定碱基序列的寡核苷酸的能力 在过去的五年里彻底改变了分子生物学领域 好几年了。这一说法的推论是，合成DNA具有 成为分子生物学家的重要和必不可少的工具。 合成的DNA可以用作探针，从 基因库，以合成新基因，为 将定点突变引入蛋白质中，并 对大片未鉴定的DNA进行快速测序。十 加州州立大学独立资助的USPHS支持的研究项目。 有立即和持续的服务需求- 校园DNA合成设施。 没有校园内的DNA合成设施的事实是 CSU强烈削弱CSU教职员工使用 作为研究工具的寡核苷酸我们建议纠正这一点 通过购买DNA合成器和 辅助设备。将组织计划提交给 有效使用和维护所需的设备。

The ability to synthesize oligonucleotides of defined base sequence has revolutionize the field of molecular biology over the past five years. A corollary to this statement is that synthetic DNA has become an important and essential tool for the molecular biologist. Synthetic DNAs can be used as probes to isolate specific genes from gene libraries, to synthesize new genes, to prepare templates for the introduction of site directed mutations into proteins, and to rapidly sequence large tracts of uncharacterized DNA. Ten independently-funded USPHS-supported research programs at C.S.U. have an immediate and continuing need for the services of an on- campus DNA synthesis facility. The fact that there is no on-campus DNA synthesis facility at C.S.U. strongly detracts from the ability of C.S.U. faculty to use oligonucleotides as research tools We propose to remedy this situation through the acquisition of a DNA synthesizer and ancillary equipment. An organizational plan is presented to effectively utilize and maintain the requested equipment.