ANTIGENIC ANALYSIS OF SEXUAL STAGES OF MALARIA PARASITES

存在于配子和动子表面的三种蛋白质 被单抗识别为抗体的目标 阻止配子细胞对蚊子的感染性。我们克隆了一个 通过免疫亲和力纯化这些蛋白质， 对该蛋白的胰酶多肽进行微测序，然后筛选 含寡核苷酸的基因组文库。净化的方法 微测序的其他目标蛋白目前正在 发展起来的。我们一直在继续寻找新的目标抗原 阻断性期疟疾传播的抗体 寄生虫，并已鉴定出一种寄生虫分子，可能是 参与红细胞中配子细胞的释放。

Three proteins present on the surface of gametes and ookinetes have been identified by monoclonal antibodies as targets of antibodies that block infectivity of gametocytes to mosquitoes. We cloned one of these proteins by immunoaffinity purifying the protein, microsequencing tryptic peptides of the protein, and then screening genomic libraries with oligonucleotides. The methods for purifying the other target proteins for microsequencing are currently being developed. We have continued the search for novel target antigens of antibodies that block transmission of sexual stage malaria parasites, and have identified a parasite molecule that may be involved in release of gametocytes from red blood cells.