DEFINITION OF HUMAN HISTOCOMPATIBILITY ANTIGENS

使用T细胞识别（主要是细胞介导的裂解），我们有 继续探索T细胞识别HLA的复杂性， 同种抗原 我们采用了一种新颖的方法， 细胞毒性T淋巴细胞（CTL）克隆作为选择剂， 富集ICR-191诱变的淋巴母细胞样细胞系（LCL） 这些细胞已经失去了被克隆细胞裂解的能力。 使用 这种方法，已经衍生出24种突变LCL， 被“选择性”DPw 2同种特异性CTL克隆裂解。 血清学 分析证实了DPw 2的丢失，并且Nopletin印迹分析 显示在许多组织中DP α或DP β mRNA的丢失， 变种人 这些突变体随后被用作 证明DP α要求的信息探针 和DP β被其他puerectin“DPw 2-特异性”CTL识别 具有更复杂特异性的克隆。 其中的特异性 克隆表明它们可以识别 另一种HLA基因以DP限制的方式。

Using T cell recognition (primarily cell-mediated lysis) we have continued to probe the complexities of T cell recognition of HLA alloantigens. We have pursued the novel approach of using cytotoxic T lymphocyte (CTL) clones as the selective agent to enrich for ICR-191 mutagenized lymphoblastoid cell lines (LCL) which have lost their capacity to be lysed by that clone. Using that approach, 24 mutant LCL have been derived which are not lysed by the "selecting" DPw2-allospecific CTL clone. Serological analysis confirms the loss of DPw2 and Nothern blot analysis demonstrates loss of DP alpha or DP beta mRNA in many of the mutants. These mutants have subsequently been used as informative probes to demonstrated the requirement for DP alpha and DP beta recognition by other putatively "DPw2-specific" CTL clones with more complex specificity. The specificity of these clones suggests that they may recognize peptide fragments of another HLA gene(s) in a DP-restricted fashion.