BIOLOGIC PROTERTIES OF NUCLEAR ONCOGENES

分子生物学在上皮细胞中的应用进展 癌症导致了特定遗传病变的鉴定， 导致关键靶基因的激活或失活。 这些基因，称为癌基因，参与了癌症的各个方面。 调节细胞生长，因此在早期 致癌过程的“启动”和“促进”。 现在 关键是要了解这些基因 因此最终可以衍生出分子或药理学试剂 来改变或抑制它们的作用。 我们选择探索2的生物学和生化功能， 显性癌基因（L-myc和c-jun）。 转录和翻译 L-myc的产物可以表征并与生物学特性相关。 功能协调发展的 此外，c-myc和L-myc之间的杂合分子具有 这两个家族的不同之处 成员 最终，这些基因的结构-功能分析将是 测定 同样，我们最近描述了c-jun的转化功能 在哺乳动物细胞中，现在已经通过删除绘制了该功能 突变 对转化和反式激活重要的区域 包括N-末端反式激活结构域、DNA结合区， 亮氨酸拉链结构域。 此外，c-jun与Jun B的比较 将开展反式激活和转化活动， 了解这些基因之间的差异。

Recent developments in application of molecular biology to epithelial cancers have led to the identification of specific genetic lesions resulting in either activation or inactivation of key target genes. These genes, called oncogenes, are involved in various aspects in the regulation of cell growth and as such play major roles in the early carcinogenic processes of "initiation" and "promotion". It is now critical to understand the precise mechanism by which these genes function so molecular or pharmacologic agents can ultimately be derived to alter or repress their effects. We have chosen to explore the biologic and biochemical functions of 2 dominant oncogenes (L-myc and c-jun). Transcriptional and translational products of L-myc may been characterized and correlated with biologic functions. In addition, hybrid molecules between c-myc and L-myc have been made to characterize the differences between these two family members. Ultimately, structure-function analysis for these genes will be determined. Likewise, we have recently described the transforming function of c-jun in mammalian cells and have now mapped this function by deletion mutation. Regions important for transformation and transactivation include an N-terminal transactivation domain, DNA binding region, and the leucine zipper domain. In addition, comparisons of c-jun with Jun B transactivation and transformation activities will be undertaken to understand the difference between these genes.