STRUCTURE AND BIOSYNTHESIS OF PROTEOGLYCANS

该项目的目的是研究化学和物理 许多组织和细胞中蛋白聚糖的性质和代谢 系统. 目前感兴趣的主题包括：1）蛋白质化学和 Swarm大鼠蛋白多糖核心蛋白的免疫学研究 布雷菲德菌素A对核心蛋白生物合成的影响 前体和成熟蛋白聚糖的加工; 3）硫酸化的分析 寡糖对骨和软骨中蛋白聚糖和唾液酸蛋白的影响 氯酸盐对骨唾液蛋白硫酸化的影响 甲状旁腺激素对骨肉瘤细胞系的影响 骨细胞合成透明质酸和蛋白多糖。

The purpose of the project is to study the chemical and physical properties and metabolism of proteoglycans in a number of tissue and cell systems. Topics of present interest include: 1) Protein chemistry and immunology of the core protein of proteoglycans from the Swarm rat chondrosarcoma; 2) Effects of brefeldin A on biosynthesis of core protein precursors and processing to mature proteoglycans; 3) analyses of sulfated oligosaccharides on proteoglycans and sialoproteins in bone and cartilage tumor cells; 4) Effects of chlorate on sulfation of bone sialoprotein in a bone osteosarcoma cell line; 5) Effects of parathyroid hormone on hyaluronic acid and proteoglycan synthesis by bone cells.