MECHANISMS OF NEUROENDOCRINE REGULATION

研究主要集中在下丘脑的调节机制上。 以及应激时的脑下垂体功能。冷应激期间的研究表明 血浆促肾上腺皮质激素一过性升高和持续升高 皮质酮，类似于对束缚应激的反应。在……里面 与制动相比，CRH受体在前部没有变化。 垂体，但中间叶明显增多。冷应激 大鼠血浆中的中间垂体肽，CLIP， 与对照组相似，但对CRH注射的反应减少，尽管 CRH受体增加。冷注射CRH引起的血浆ACTH反应 暴露大鼠与对照组相似，但对5分钟的反应 乙醚暴露增加，表明对一种新的 应激依赖于下丘脑释放额外的因子，这些因子 协同CRH的作用。冷应激大鼠表现出更大的下降 在任何一种压力下，正中隆起的CRH含量，表明 慢性应激时下丘脑反应的高敏感性。 对中间叶垂体多巴胺能调节的研究表明 CRH受体浓度呈时间依赖性增加，尽管显著 细胞萎缩，POMC基因表达水平下降。比如在寒冷的时候 应激，CRH受体上调并不伴随平行 功能变化。 开始研究以确定下丘脑释放的调节 用免疫组织化学方法检测CRH和VP的表达。一项新发现是 α-1肾上腺素能受体是应激诱导的重要介质 下丘脑室旁核CRH的合成及其在正中隆起的释放。研究 正在进行中，以阐明多重的生理作用 神经递质参与调节下丘脑CRH和VP。

Investigation has focused on the mechanism of regulation of hypothalamic and pituitary function during stress. Studies during cold stress show transient increases in plasma ACTH and sustained increases in corticosterone, similar to the responses to immobilization stress. In contrast to immobilization, CRH receptors were unchanged in the anterior pituitary, but markedly increased in the intermediate lobe. Cold-stressed rats showed plasma levels of the intermediate pituitary peptide, CLIP, similar to controls, but reduced responses to CRH injection, despite the increase in CRH receptors. Plasma ACTH responses to CRH injection in cold exposed rats were similar to those in controls, but the response to 5 min ether exposure was enhanced, indicating that hypersensitivity to a novel stress depends upon hypothalamic release of additional factors that synergise the effect of CRH. Cold-stressed rats showed larger decreases in CRH content in the median eminence following either stress, indicating the hypersensitivity of the hypothalamic response during chronic stress. Studies on the dopaminergic regulation of the intermediate pituitary showed time dependent increases in CRH receptor concentration, despite marked cellular atrophy and decreases in POMC mRNA levels. As during cold stress, CRH receptors upregulation was not accompanied by parallel functional changes. Studies were initiated to determine the regulation of hypothalamic release of CRH and VP, using immunohistochemical techniques. An new finding was that alpha-1 adrenergic receptors are important mediators of stress-induced CRH synthesis in the PVN and its release from the median eminence. Studies are in progress to elucidate the physiological role of the multiple neurotransmitters involved in the regulation of hypothalamic CRH and VP.