SITE-DIRECTED MUTAGENESIS OF NONMUSCLE MYOSIN HEAVY CHAINS

先前发现的编码鸡脑头部区域的3.7kbpc DNA 非肌肉肌球蛋白重链(脊椎动物非肌肉肌球蛋白的B亚型 II重链)被插入昆虫载体pBlueBac中，并将 以表达镁含量为目的的转基因昆虫细胞 肌球蛋白重链。我们的目标是恢复一个完全活跃的非肌肉 肌球蛋白重链使我们可以研究肌球蛋白轻链的作用 链对酶活性的影响及插入氨基的影响 我们最近在肌球蛋白头部发现的酸性序列 从鸡脑文库中克隆的cdna。

3.7 kbp of cDNA previously found to encode the head region of chicken brain nonmuscle myosin heavy chain (a B isoform of vertebrate nonmuscle myosin II heavy chain) was inserted into the insect vector pBlueBac and will be transfected into insect cells with the purpose of expressing mg quantities of the myosin heavy chain. Our goal is to recover a fully active nonmuscle myosin heavy chain so that we can examine the role of the myosin light chains on enzymatic activity as well as the effect of the inserted amino acid sequences we have recently discovered in the head portion of myosin cDNA cloned from a chicken brain library.