INTERACTIVE TOXICITY--SUPERFUND HAZARDOUS SUBSTANCES

该提案的重点是制定检测、评估和 了解环境中有害物质的毒性， 垃圾场，并加强细菌降解作为一种 减少有害物质的数量和毒性的程序。 这 focus利用了我们在毒理学方面现有的研究优势， 环境微生物学、分析化学、环境 工程和水文学。 我们寻求了解从一个地点迁移的化学物种， 它们是基于与细菌的相互作用而形成的。 的 这些化学物质的特性鉴定将需要合作 在水文学和化学项目之间，特别是在 开发选择性和灵敏度分析， 有机物种。 这些研究结果将用于指导毒理学 实验 提出了确定交互毒性的基础研究 金属（类金属）之间和重要的有机物种之间， 无机物 这与一个项目相协调，以评估重要的 解毒这些金属（类金属）的细胞机制和重要因素 从而改变这些机制。 其他项目研究免疫和 对环境污染物的致畸反应。 提高细菌效用以补救或减少 环境毒性的毒性。 的退化 卤化烃和细菌可以完成这些 反应需要检查。 细菌的运动对于 了解细菌分布，并计划如何有益于 细菌可以有效地应用于受污染的场地。 这将 除了研究提高垃圾处理率的场地处理方案外， 有害垃圾场化学品的细菌降解。

The focus f this proposal is to develop methods to detect, evaluate and understand the toxicity of hazardous substances tin the environment of dump sites and to enhance the utility of bacterial degradation as a procedure to reduce the amount and toxicity of hazardous substances. This focus takes advantage of our existing research strengths in toxicology, environmental microbiology, analytical chemistry, environmental engineering and hydrology. We seek to understand the chemical species which migrate from a site and which are formed based on interactions with bacteria. The characterization of these chemical species will require cooperation between projects in hydrology and chemistry, particularly in the development of selective and sensitive analyses to quantitate both metal and organic species. The results from these studies will be used to quide the toxicology experiments. Basic reseach is proposed to determine interactive toxicity between metal(loid)s and between important organic species and the inorganics. This is coordinated with a project to evaluate the important cellular mechanisms to detoxify these metal(loid)s and important factors which modify these mechanisms. Additional projects investigate immune and teratogenic responses to environmental contaminants. Projects to enhance the utility of bacteria to remediate or reduce the toxicity of environmental toxicity are proposed. The degredation of halogenated hydrocarbons and the bacteria which can accomplish these reactions are to be examined. The movement of bacteria are important in understanding bacterial distribution and in planning how beneficial bacteria could be effectively applied to a contaminated site. This will be studied in addition to site treatment schemes which enhance the rates of bacterial degredation of hazardous dumpsite chemicals.