HAZARDOUS SUBSTANCES BASIC RESEARCH PROGRAM

加拿大多伦多大学有害物质基础研究计划 辛辛那提是一个由医学院教师组成的联盟， 工程、艺术和科学，其主要目标是开发新的 方法，将是有用的，在有毒有机物的微生物降解， 危险废物场和工业废水中发现的物质。 本提案中有三个基础研究领域， 生物医学部分包括三个项目，以调查 三类化学废物的生物降解和毒理学。 在 项目1分子遗传学将用于基因工程， 能代谢环境有害物质的微生物 多氯芳烃 项目2将涉及 开发微生物系统以好氧降解偶氮染料， 将染料转化为无害产品。 本项目后期 将涉及基因操作和微生物修饰， 优化解毒。 项目3涉及微生物降解 具有致癌性的4环和5环多环和N-杂环 芳香烃. 该项目的研究将涉及化学物质 降解的毒性特性的表征和测定 产品. 工程部分，项目4，是针对 新型高效微生物生物反应器的设计与评价 降解 该项目涉及有氧和无氧的评价 方法，即中空纤维单元，螺旋缠绕膜组件和 用于有害化合物生物降解的生物膜。 的一个主要方面 该项目将调查现有的生态相互关系， 和基因增强的微生物 污染物的生物降解。 仪器组件，项目5， 将涉及电感耦合等离子体质量的进一步发展 超痕量毒物检测光谱法。 该项目将侧重于开发在全球范围内的检测方法。 毫微微克到微微克级别的含氯和含硫化合物 感兴趣的生物医学和工程组件。 其他 还将研究危险废物场地中感兴趣的化合物， 尤其是金属。

The Hazardous Substances Basic Research Program at the University of Cincinnati is a consortium of faculty of the Colleges of Medicine, Engineering, and Arts and Science with the primary goal of developing new methods that will be useful in the microbial degradation of toxic organic substances found in hazardous waste sites and industrial effluents. There are three areas of basic research interests in this proposal, a Biomedical component consists of three projects to investigate the biodegradation and toxicology of three classes of chemical wastes. In Project 1 molecular genetics will be used in the gene engineering of microbes capable of metabolizing environmentally recalcitrant polychlorinated aromatic hydrocarbons. Project 2 will involve the development of microbial systems to aerobically degrade azo dyes and related dye stuffs to innocuous products. Later phases of this project will involve genetic manipulation and modification of microbes to optimize detoxification. Project 3 deals with the microbial degradation of recalcitrant carcinogenic 4 and 5 ring polycyclic and N-heterocyclic aromatic hydrocarbons. Studies in this project will involve the chemical characterization and determination of toxic properties of the degradation products. The Engineering component, Project 4, is directed toward the design and evaluation of novel bioreactors for effective microbial degradation. This project involves evaluation of aerobic and anaerobic methods, i.e. hollow-fiber units, spiral-wound membrane modules and biofilms for biodegradation of hazardous compounds. A major aspect of this project will investigate the ecologic interrelationships in existing and genetically enhanced microbes in communities during the biodegradation of pollutants. The Instrumentation component, Project 5, will involve the further development of Inductively-Coupled Plasma-Mass Spectrometry for the detection of toxicants at the ultra-trace levels. This project will focus on development of methods of detection at the femtogram to picogram levels of chlorine and sulfur containing compounds of interest to the Biomedical and Engineering components. Other compounds of interest in hazardous waste sites will also be studied, particularly metals.