BIOLOGICAL HAZARDOUS WASTE MANAGEMENT

提案方案的总体目标是寻求减少 与环境污染物相关的生物风险 这些废物的处理。白色腐菌木质素降解系统 将用于提出了一个综合方案，从分子 生物学和遗传学，从酶学到工程学 和应用研究。木质素酶的基因将是 用于大规模生产的表达系统中， 内切酶真核表达系统是今后研究的重点 将木质素酶基因掺入到原始真菌中， 提高机体的能力。重组和天然 酶的特征在于其物理性质、周转率 速率、底物特异性和稳定性。机制和途径 将表征降解，以确定是否存在潜在的有害 酶和真菌可能会产生中间产物。条件 将寻求最大限度地提高退化率和程度， 生产可能有害的产品。最后，我们将研究 过程的工程设计及其成本。该计划将成为 并部分支持犹他州州立大学的两个研究生项目， 毒理学、分子生物学和生物化学研究生课程。 该研究计划将涉及化学系的教师 生物化学、生物学、营养学和食品科学，以及民用和 犹他州州立大学环境工程系 宾夕法尼亚州立大学的分子和细胞生物学。研究 生物技术中心和犹他州水资源研究中心也将参与其中 实验室这个项目的意义在于我们发现了一个 环境污染物生物氧化的自然过程。我们 建议调整系统以适应问题，并研究如何增加 其有效性。应该可以设计得非常经济 以木屑、玉米棒、花生壳 小麦秸秆等，这取决于这些物质的可用性。 其中任何一个都将提供一个经济而又非常有效的系统 因为这些白色腐烂真菌会与 营养素

The overall objective of the proposal program is to seek ways to decrease the risks associated with environmental pollutants by biological treatment of such wastes. The lignin degrading system of white rot fungi will be used. An integrated program is proposed, starting with molecular biology and genetics and proceeding from enzymology through engineering and application research. The genes for the ligninases will be incorporated into expression systems for large-scale production of enzymes. Eukaryotic expression systems will be studied for future incorporation of the ligninase genes back into the original fungus to increase the capacity of the organism. Both recombinant and native enzymes will be characterized in terms of physical properties, turnover rates, substrate specificity, and stability. The mechanisms and pathways for degradation will be characterized to determine if potential harmful intermediates might be produced by the enzymes and the fungus. Conditions will be sought to maximize rates and extents of degradation to minimize the production of potentially harmful products. Finally we will study the engineering of the process and its cost. The program will be a part of and partially support two graduate programs at Utah State University, the Graduate Programs in Toxicology and Molecular Biology and Biochemistry. The research program will involve faculty in the Departments of Chemistry and Biochemistry, Biology, Nutrition and Food Sciences, and Civil and Environmental Engineering at Utah State University and the Department of Molecular and Cell Biology at Pennsylvania State University. The research will also involve the Biotechnology Center and the Utah Water Research Laboratory. The significance of this project is that we have discovered a natural process for biological oxidation of environmental pollutants. We propose to adapt the system to the problem and to study how to increase its effectiveness. It should be possible to design very economical systems based on nutrients such as sawdust, corn cobs, peanut shells, wheat straw, etc. depending upon the availability of these substances. Any one of these would provide an economical yet very effective system because the white rot fungi would be very competitive with such nutrients.