FINNEGAN MATT SSQ-700 WITH ADDITIONAL DATA SYSTEM

毛细管气相色谱-负离子化学电离联用 质谱学提供和定量低水平的 前列腺素、白三烯、血小板活化因子 羟基二十烷、酮二十烷和甘油二酯的络合物 生物体液。请拨资金购买一辆芬兰车 MAT SSQ-700R单级四极GC/MS/DS，附加数据 系统8-Mb RAM扩展和两个降压/升压变压器(获取 费用--264,700美元)，由10名合作调查员分担。这个 检测将用于为以下各项提供专用检测 毛细管气相色谱-负离子化学电离联用 质谱学技术对确定其作用至关重要 人类哮喘、过敏、肺部疾病和其他疾病中的脂质介质 肺部损伤。 前列腺素，白三烯B4，羟基二十烷类， 二十烷基酮和甘油二酯直接转化为 具有合适气相性质的电子捕获衍生物 与多氟试剂反应简便。硫磺多肽- 白三烯(白三烯C4、D4和E4)和PAF是化学上的 在合成电子俘获衍生物之前进行了修饰。这个 除去白三烯C4、D4和E4的硫多肽基团，留下 二十烷类骨架，然后转化为五氟苯甲基 埃斯特。PAF的磷酸酯在合成前先进行水解 二甘油酯五氟苯甲酸酯衍生物。精致的 组合毛细管的灵敏度(检测限&lt；100 fg/进样) 气相色谱-负离子化学电离质谱仪 已导致对简化的和不那么劳动密集型的 比以前在质谱学中要求的提纯程序 化验。反过来，更完整的脂类分布的测定 生物样品中的介体是可以获得的。建议数 工具应该为更合理的方法提供信息 在预防、管理和治疗哮喘、过敏、 肺部疾病和肺损伤。

Combined capillary gas chromatography-negative ion chemical ionization mass spectrometry provid and quantitation of low-levels of prostaglandins, leukotrienes, platelet-activating factor (PAF), hydroxy-eicosanoids, keto-eicosanoids and diacylglycerides in complex biological fluids. Funds are requested for the purchase of a Finnigan MAT SSQ-700R single stage quadrupole GC/MS/DS with additional data system 8-Mb RAM expansion and two buck/boost transformers (acquisition cost - $264,700) to be shared by ten co-investigators. The instrumentation will be used to provide dedicated instrumentation for combined capillary gas chromatography-negative ion chemical ionization mass spectrometric techniques critical for determination of the role of lipid mediators in human asthma, allergy, pulmonary disease and pulmonary injury. The prostaglandins, leukotriene B4, hydroxy-eicosanoids, keto-eicosanoids and the diacylglycerides are directly converted to electron-capture derivatives with suitable vapor phase properties via facile reaction with multifluorinated reagents. The sulfidopeptido- leukotrienes (leukotrienes C4, D4 and E4) and PAF are chemically modified prior to synthesis of electron-capture derivatives. The sulfidopeptido group of leukotrienes C4, D4 and E4 is removed leaving an eicosanoid skeleton which is then converted to the pentafluorobenzyl ester. The phosphate ester of PAF is hydrolyzed prior to synthesis of the diglyceride pentafluorobenzoyl ester derivative. The exquisite sensitivity (detection limits < 100 fg/injection) of combined capillary gas chromatography-negative ion chemical ionization mass spectrometry has resulted in the adaptation of simplified and less labor-intensive purification procedures than previously required in mass spectrometric assays. In turn, more complete determinations of the profiles of lipid mediators in biological samples are obtainable. The proposed instrumentation should provide information for more rational approaches in the prevention, management and treatment of asthma, allergy, pulmonary disease and pulmonary injury.