COMPUTER FACILITY FOR RAPID 3-D IMAGING

对复杂的生物系统在结构的各个层次上的大量研究 需要快速计算三维结构 以及它们的可视化和真实的操作。 结构数据 在核心用户群的分子和细胞水平上， 主要来自电子显微镜，而活细胞的图像 通过荧光显微镜获得;器官和组织的数据 水平是从生活中的人计算的技术， 断层扫描（CT）和磁共振成像（MRI）。 VAX服务器 3900、X-windows终端以及Stardent超级图形计算机 设施的所有用户都需要增强功能， 数据的计算和可视化。 Optronics接口是 需要对现有的Optronics显微密度计数据进行数字化。 荧光显微镜工作站、CCD和SIT摄像机， 是从活细胞中收集数据所必需的。 核心用户 小组由来自六个不同部门的14名科学家组成， 研究机构开展10个研究项目。 研究的主题 主要使用者多种多样：肌丝结构粗，包括 骨干和跨桥互动，发展动态和其他 肌肉和非肌肉细胞的变化，兴奋-收缩偶联， 视网膜回路结构和功能，癌基因在生长中的作用 分化、肺泡液摄取、纤维蛋白原和纤维蛋白 结构和组装，以及通过CT对解剖结构成像， 核磁共振 大多数核心用户都有一定的计算机图像处理经验 处理和图形应用于生物问题，并有研究 受当前可用资源限制的项目 仪器仪表 所要求的设备将打开令人兴奋的新 研究的可能性，为各种各样的科学家在 宾夕法尼亚大学。

Much research on complex biological systems at all levels of structure shares the need for rapid calculation of three dimensional structures and their visualization and manipulation in real time. Structural data at the molecular and cellular levels of the core user group are derived primarily from electron microscopy, while images of living cells are obtained by fluorescent light microscopy; data at the organ and tissue level are derived from living people by the techniques of computed tomography (CT) and magnetic resonance imaging (MRI). The VAXserver 3900, X-windows terminals as well as the Stardent supergraphics computer with enhancements are needed by all users of the facility for computation and visualization of data. The Optronics interface is needed for digitizing data on the existing Optronics microdensitometer. The fluorescent light microscope workstation and CCD and SIT cameras and are necessary for collecting data from living cells. The core user group consists of 14 scientists from six different departments or institutes pursuing ten research projects. The subjects studied by the major users are diverse: muscle thick filament structure, including the backbone and crossbridge interactions, dynamics of development and other changes in muscle and non-muscle cells, excitation-contraction coupling, structure and function of retinal circuitry, role of oncogenes in growth and differentiation, alveolar fluid uptake, fibrinogen and fibrin structure and assembly, and imaging of anatomical structures by CT and MRI. Most of the core users have some experience with computer image processing and graphics applied to biological problems and have research programs that have been limited by the currently available instrumentation. The requested equipment will open exciting new research possibilities for a wide variety of scientists at the University of Pennsylvania.