Scanning Electron Microscope

扫描电子显微镜

• 批准号: 8639772
• 负责人: JOHN W WEISEL
• 金额: $ 38.18万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-05-15 至 2015-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8639772
• 关键词: 18 year old; Address; Back; Biochemistry; Biophysics; Cell surface; Core Facility; Dehydration; Detection; Developmental Cell Biology; Electron Microscope; Electron Microscopy; Electrons; Ensure; Faculty; Funding; Gold Colloid; Grant; Image; Investments; Laboratories; Medicine; Metals; Microscope; Microscopy; Mission; Morphologic artifacts; Pennsylvania; Productivity; Research; Research Personnel; Research Project Grants; Resolution; Resources; Sampling; Scanning; Scientist; Specimen; Structure; System; Training; United States National Institutes of Health; Universities; detector; instrument; instrumentation; light microscopy; macromolecule; medical schools; particle; quantum

DESCRIPTION (provided by applicant): NIH-funded researchers at the University of Pennsylvania request funds for a shared instrumentation grant to replace an 18-year old scanning electron microscope (SEM) that has been a workhorse for the School of Medicine (SOM). Despite the best efforts and significant investment of both our faculty and staff and the microscope company, this only SEM available to Penn Medicine researchers has been unreliable for the past few years and has recently become practically unusable. The aim of this proposal is to provide a FEI Quanta 250 microscope that will serve the basic needs for the examination of traditional dehydrated, metal coated samples with SEM, but also including the capability for correlative light microscopy-SEM, imaging of hydrated samples, and back-scattered electron detection of colloidal gold particles. This instrument will support a wide range of research throughout the SOM and across the campus. The studies described here require the substantially enhanced imaging capabilities of this system for NIH-funded research projects. Specifically, all investigators use various types of light microscopy in their research and require the ability to correlate the images obtained in this manner with higher resolution SEM images. Most of the scientists also require the identification and localization of specific macromolecules on the surface of cells or other structures, which requires a back-scattered electron detector. Because of the well-known artifacts of dehydration and metal coating, the ability to image hydrated specimens by environmental SEM is now necessary. We expect that demand and usage for the SEM will continue to rise as a consequence of these new capabilities. An indication of the very strong and broad support for this SEM is the commitment of substantial matching funds from the Department of Cell and Developmental Biology, together with the inclusion of the instrument in the Electron Microscopy Resource Laboratory, with core support and administration by the Department of Biochemistry and Biophysics, and the recognition and support of the SOM administration that SEM is a strategic necessity. This new microscope will allow fundamental biomedical problems to be addressed that would otherwise not be possible, will ensure continued productivity from the researchers, and will maintain the microscopy-training mission of our core facilities.

描述(由申请人提供)：美国国立卫生研究院资助的宾夕法尼亚大学的研究人员申请资金，以共享仪器拨款，以更换已有18年历史的扫描电子显微镜(SEM)，这一直是医学院(SOM)的主力。尽管我们的教职员工和显微镜公司付出了最大的努力和巨大的投资，但在过去的几年里，宾夕法尼亚大学医学研究人员唯一可用的扫描电子显微镜一直不可靠，最近几乎无法使用。这项提议的目的是提供一台FEI Quanta250显微镜，它将满足传统的脱水金属涂层样品的扫描电子显微镜检查的基本需求，同时还包括相关光学显微镜-扫描电子显微镜、水合样品的成像以及胶体金粒子的背散射电子检测。这台仪器支持的范围很广 在整个SOM和整个校园的研究。这里描述的研究需要为NIH资助的研究项目大大增强该系统的成像能力。具体地说，所有研究人员在他们的研究中都使用了各种类型的光学显微镜，并要求 将以这种方式获得的图像与更高分辨率的扫描电子显微镜图像进行关联的能力。大多数科学家还需要在细胞或其他结构的表面识别和定位特定的大分子，这需要背向散射电子探测器。由于众所周知的脱水和金属涂层的人工制品，现在需要通过环境扫描电子显微镜对水合样品进行成像的能力。我们预计，由于这些新功能，对扫描电子显微镜的需求和使用量将继续上升。细胞和发育生物学系承诺提供大量的配套资金，并将该仪器纳入电子显微镜资源实验室，由生物化学和生物物理系提供核心支持和管理，这表明扫描电子显微镜得到了非常有力和广泛的支持，以及SOM管理部门承认和支持扫描电子显微镜是一种战略需要。这一新型显微镜将解决原本不可能解决的基本生物医学问题，将确保研究人员持续提高工作效率，并将维持我们核心设施的显微镜培训任务。