Scanning Electron Microscope

扫描电子显微镜

• 批准号: 8639772
• 负责人: JOHN W WEISEL
• 金额: $ 38.18万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-05-15 至 2015-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8639772
• 关键词: 18 year old; Address; Back; Biochemistry; Biophysics; Cell surface; Core Facility; Dehydration; Detection; Developmental Cell Biology; Electron Microscope; Electron Microscopy; Electrons; Ensure; Faculty; Funding; Gold Colloid; Grant; Image; Investments; Laboratories; Medicine; Metals; Microscope; Microscopy; Mission; Morphologic artifacts; Pennsylvania; Productivity; Research; Research Personnel; Research Project Grants; Resolution; Resources; Sampling; Scanning; Scientist; Specimen; Structure; System; Training; United States National Institutes of Health; Universities; detector; instrument; instrumentation; light microscopy; macromolecule; medical schools; particle; quantum

DESCRIPTION (provided by applicant): NIH-funded researchers at the University of Pennsylvania request funds for a shared instrumentation grant to replace an 18-year old scanning electron microscope (SEM) that has been a workhorse for the School of Medicine (SOM). Despite the best efforts and significant investment of both our faculty and staff and the microscope company, this only SEM available to Penn Medicine researchers has been unreliable for the past few years and has recently become practically unusable. The aim of this proposal is to provide a FEI Quanta 250 microscope that will serve the basic needs for the examination of traditional dehydrated, metal coated samples with SEM, but also including the capability for correlative light microscopy-SEM, imaging of hydrated samples, and back-scattered electron detection of colloidal gold particles. This instrument will support a wide range of research throughout the SOM and across the campus. The studies described here require the substantially enhanced imaging capabilities of this system for NIH-funded research projects. Specifically, all investigators use various types of light microscopy in their research and require the ability to correlate the images obtained in this manner with higher resolution SEM images. Most of the scientists also require the identification and localization of specific macromolecules on the surface of cells or other structures, which requires a back-scattered electron detector. Because of the well-known artifacts of dehydration and metal coating, the ability to image hydrated specimens by environmental SEM is now necessary. We expect that demand and usage for the SEM will continue to rise as a consequence of these new capabilities. An indication of the very strong and broad support for this SEM is the commitment of substantial matching funds from the Department of Cell and Developmental Biology, together with the inclusion of the instrument in the Electron Microscopy Resource Laboratory, with core support and administration by the Department of Biochemistry and Biophysics, and the recognition and support of the SOM administration that SEM is a strategic necessity. This new microscope will allow fundamental biomedical problems to be addressed that would otherwise not be possible, will ensure continued productivity from the researchers, and will maintain the microscopy-training mission of our core facilities.

描述（由申请人提供）：宾夕法尼亚大学NIH资助的研究人员请求资金共享仪器赠款，以取代18岁的扫描电子显微镜（SEM），该扫描电子显微镜（SEM）一直是医学院（SOM）的主力军。尽管我们的教职员工和显微镜公司都做出了最好的努力和大量投资，但在过去的几年中，Penn Medicine研究人员可用于宾夕法尼亚医学研究人员的这种SEM一直是不可靠的，并且最近几乎变得无法使用。该提案的目的是提供FEI量子250显微镜，该显微镜将满足检查带有SEM的传统脱水，金属涂层样品的基本需求，还包括相关光显微镜SEM的能力，水合样品的成像以及胶体金颗粒的背部分散电子检测。该乐器将支持广泛的范围 整个SOM和整个校园的研究。此处描述的研究要求该系统具有大幅增强的成像能力，用于NIH资助的研究项目。具体而言，所有研究人员在研究中使用各种类型的光学显微镜，需要 将以这种方式获得的图像与较高分辨率SEM图像相关联的能力。大多数科学家还需要在细胞或其他结构表面鉴定和定位特定的大分子，这需要反向散发电子检测器。由于众所周知的脱水和金属涂料的伪像，现在有必要通过环境SEM对水合样品进行成像。我们预计，由于这些新功能，SEM的需求和使用率将继续上升。对该SEM的非常有力和广泛的支持的指示是，来自细胞和发育生物学系的实质性匹配资金的承诺，以及将仪器纳入电子显微镜资源实验室中，以及生物化学和生物物理学系的核心支持和管理，以及对SOM管理的认可和支持，SOM的管理是SEM是一种战略性的必要性。这种新的显微镜将允许解决原本是不可能的基本生物医学问题，并确保研究人员的持续生产力，并保持我们核心设施的显微镜训练任务。