500 MHZ NMR INSTRUMENTATION FOR SHARED USE

高场（500 MHz）多核谱仪是满足 约翰霍普金斯大学霍姆伍德校区用户群的需求 大学化学系和生物系。 一系列 化学和生物学意义的项目描述：（1） 黄曲霉毒素和β-内酰胺抗生素的生物合成研究， 加利车霉素与寡核苷酸的相互作用。(2)合成和 低聚糖、糖肽和 糖脂用于研究碳水化合物识别和肝脏 受体结合研究。(3)金属结合的结构研究 “锌指”型肽和相关的逆转录病毒蛋白。（四） 研究RNA折叠和RNA-蛋白质相互作用。（五） 合成新方法的开发及其在全氟辛烷磺酸中的应用 不对称天然产物合成。(6)氧气的化学模拟- 结合和氧活化铜蛋白活性位点， 谷氨酰-半胱氨酰的金属结合性质的研究。 缩氨酸(7)DNA分子和寡核苷酸的结构研究 来了解DNA弯曲的起源。（八） 通过NMR技术鉴定α- 胰凝乳蛋白酶用富含13 C的酶激活抑制剂抑制。 化学和生物发光反应机制的研究。 项研究 脱氨基形成的碳正离子的低温NMR。（九） 受阻和变形烯烃的氧化和反应， 将研究卡宾的重排。(10)合成 野牡丹素及其氮杂类似物。(11)的机理研究 人胎盘芳香化酶抑制剂的作用和设计， 羊毛甾醇14 α-脱甲基酶。

A high field (500 MHz), multinuclear spectrometer is requested to meet the needs of a user group at the Homewood campus of The Johns Hopkins University from the Departments of Chemistry and Biology. A range of projects of chemical and biological significance is described: (1) Biosynthetic studies of aflatoxins and of Beta-lactam antibiotics, interactions of calicheamicin with oligonucleotides. (2) Synthetic and structural investigations of oligosaccarides, glycopeptides and glycolipids for research in carbohydrate recognition and hepatic receptor binding studies. (3) Structural studies of metal-binding peptides of the "zinc finger" type and related retroviral proteins. (4) Investigation of RNA folding and RNA-protein interactions. (5) Development of new synthetic methods and their application to total asymmetric natural product syntheses. (6) Chemical modeling of oxygen- binding and oxygen-activating copper protein active sites and investigations of the metal-binding properties of glutamyl-cysteinyl . peptides. (7) Structural studies of DNA molecules and oligonucleotides with adenine tracts to understand the origins of DNA bending. (8) Identification by NMR techniques of the labeled sites of alpha- chymotrypsin inhibited with 13C-enriched enzyme-activated inhibitors. Examination of chemi- and bioluminescent reaction mechanisms. Study by low-temperature NMR of deaminatively formed carbonium ions. (9) Oxygenation of hindered and deformed olefins and the reactions and rearrangements of carbenes will be investigated. (10) Synthesis of prostaglandins and their aza-analogues. (11) Studies of the mechanism of action and design of inhibitors for human placental aromatase and lanosterol 14alpha-demethylase.