REGULATION OF SUGAR TRANSPORT AND METABOLISM IN LACTIC ACID BACTERIA

1. N（5）-（L-1-羧乙基）-L-鸟氨酸：NADP氧化还原酶基因已被克隆。 克隆到p53-ZAPII载体中。这种酶（也称为 N（5）羧乙基鸟氨酸合酶，EC 1.5.1.24），其介导 生物合成的一个家庭以前未知的氨基酸，一直是 在大肠杆菌中表达。 2. N（5）-羧乙基鸟氨酸合酶的基因已经测序， 该酶的氨基酸序列已经确定。 3.已发现N（5）-羧乙基鸟氨酸合酶基因， 编码乳链菌肽合成和蔗糖代谢的基因是连锁的， 定位在染色体转座子上 4.已使用针对纯化酶制备的多克隆抗体 测定乳酸菌中酶的分布和组成 酸性细菌 5.果糖的运输和异化机制 口腔病原体核梭杆菌已被阐明。突变体 已经通过UV诱变分离了糖转运系统缺陷的人， 并通过放射性示踪技术鉴定了生化损伤。

1.The N(5)-(L-1-Carboxyethyl)-L-ornithine:NADP oxidoreductase gene has been cloned in the lambdaZAPII vector. This enzyme, (also called N(5)carboxyethylornithine synthase, EC 1.5.1.24), which mediates the biosynthesis of a family of previously unknown amino acids, has been expressed in Escherichia coli. 2.The gene for N(5)-carboxyethylornithine synthase has been sequenced, and the amino acid sequence of the enzyme has been determined. 3.It has been found that the N(5)-carboxyethylornithine synthase gene, and the genes encoding nisin synthesis and sucrose metabolism are linked and localized to a chromosomal transposon. 4.Polyclonal antibodies prepared against the purified enzyme have been used to survey for the distribution and constitutivity of the enzyme in lactic acid bacteria. 5.The mechanism(s) involved in the transport and dissimilation of fructose by the oral pathogen, Fusobacterium nucleatum have been elucidated. Mutants deficient in sugar transport systems have been isolated by UV mutagenesis, and the biochemical lesions have been identified by radiotracer techniques.