GC-MS ANALYSIS OF PCDF BLOOD LEVELS IN CHILDREN EXPOSED IN VITRO

已经有两起多氯联苯中毒事件 联苯（多氯联苯）及其热分解产物;第一， 1968年日本，1979年台湾第二。 因为多氯联苯是一个 广泛的污染问题，这些事件已经仔细研究，因为 他们提供了唯一的机会来直接观察毒性 工作场所以外的人类体内的多氯联苯含量。 的实验室方法 对这些暴发的评估相对简单， 1968年;从那以后，分析方法有了很大的进步。 在 NIEHS流行病学分支与台湾科学家合作， 有机会检查了100多名在子宫内的儿童， 1979年中毒事件或之后 这些孩子继续 这些化学物质不能从母亲体内排出。 我们已经检查了血液和耳垢样本的2，3，7，8- 使用选定离子的四氯二苯并呋喃和六氯二苯并呋喃 以5000的质量分辨率进行监测。 仪器灵敏度 这些分析使得可以检测到50毫微微克的分析物 在绝对水平上，信噪比为CA。二十比一 考虑 考虑样品体积和回收率， 在实际样品中的检测为ca。2.4皮克/样品（2.4 万亿分之一）。 在这些样品中未观察到TCDF或HCDF。 为了验证这些结果，将使用 I-SQ混合动力概念的选定分解监测能力 质谱仪 这种技术不如高灵敏度 分辨率选择离子监测技术，但它更具有选择性。 这些结果也与RTI在2004年获得的PCB结果一致。 没有发现PCBs。

There have been two outbreaks of human poisoning by polychlorinated biphenyls (PCBs) and their thermal breakdown products; the first, in Japan in 1968, the second in Taiwan in 1979. Because PCBs are a world wide pollution problem, these episodes have been studied carefully, since they have presented the only opportunity to observe directly the toxicity of PCBs in human beings outside the workplace. Laboratory methods for the evaluation of these outbreaks were relatively unsophisticated in 1968; there has been great progress in analytical methods since. In collaboration with Taiwanese scientists, the Epidemiology Branch, NIEHS, had the opportunity to examine over 100 children who had been in utero at the time of 1979 poisoning or afterward. These children continued to be affected, since the chemicals cannot be excreted from the mother's body. We have examined blood and cerumen samples for 2,3,7,8- tetrachlorodibenzofuran and hexachlorodibenzofuran using selected ion monitoring at a mass resolution of 5,000. Instrument sensitivity during these analyses was such that 50 femtograms of analyte could be detected on an absolute level with a signal to noise of ca. 20:1. Taking into account sample volume and recovery yields, the practical limits of detection in the actual samples was ca. 2.4 picogram per sample (2.4 parts per trillion). No TCDFs or HCDFs were observed in these samples. To verify these results, selected samples will be analyzed using the selected decomposition monitoring capabilities of the concept I-SQ hybrid mass spectrometer. This technique is less sensitive than the high resolution selected ion monitoring technique but it is more selective. These results are also consistent with the PCB results obtained by RTI in which no PCBs were observed.