PROPOSAL FOR AN AUTOMATED DNA SEQUENCING FACILITY

该提案的目标是提供快速、准确、低成本的DNA 序列分析的直接研究社区的所有成员。 这 将通过建立一个 机构DNA测序设施。 使用传统方法， DNA测序技术是重复、繁琐和劳动密集型。 在 此外，该技术仅由科学家有效地执行， 在技术上非常熟练。 本建议书所述的设施 将采用一个自动化实验室工作站，2台自动DNA测序仪 和自动DNA序列胶片读取器。 提供的服务 该设施将使调查人员从许多重复性的工作中解脱出来 与DNA测序，包括测序反应的执行，数据 采集和数据解释。 自动化DNA测序 精确、快速和安全，数据生成能力是 比传统的放射性方法更高。 此外，委员会认为， 使用自动测序仪进行DNA序列分析的成本是 估计是放射性方法的十分之一。 DNA测序设施的设计是为了满足测序的需要， 一群非常多样化的研究科学家。 对于调查人员来说， 进入分子生物学领域，小规模或一次性用户， DNA测序能力的建立可能非常昂贵， 设备时间和人力 一个DNA测序设备将允许 以最小的花费获得DNA序列信息。 为 有一种强烈的愿望，那就是要做一个大的... 然而，涉及数百个DNA酶的大规模测序项目， 使用目前的人工测序技术， 不可行。 这些类型的项目将是现实的， DNA测序设备的一部分 基因的DNA序列是其最基本的特征之一。 许多关键的问题，关于结构，表达和功能的一个 基因可以用序列的知识来寻址。 的可用性 DNA测序设施的使用可以让研究人员探索 由于缺乏资源而无法进行的研究。

The objective of this proposal is to provide rapid, accurate,low-cost DNA sequence analysis to all members of the immediate research community. This objective will be accomplished through the establishment of an Institutional DNA Sequencing Facility. Using traditional methods, the technique of DNA sequencing is repetitive, tedious and labor-intensive. In addition, the technique is performed, efficiently, only by scientists, highly skilled in the technique. The facility described in this proposal will employ an automated laboratory workstation, 2 automated DNA sequencers and an automated DNA sequence film reader. The services provided by the facility will free investigators from many of the repetitive tasks involved with DNA sequencing, including execution of sequencing reactions, data acquisition and data interpretation. Automated DNA sequencing is more precise, faster and safer and the capacity for data generation is orders of magnitude higher than with traditional, radioactive methods. Furthermore, the cost of DNA sequence analysis using the automated sequencer is estimated to be one tenth the cost using radioactive methods. The DNA sequencing facility is designed to satisfy the sequencing needs of a very diverse group of research scientists. For investigators just entering the field of molecular biology, small-scale or one-time users, the establishment of DNA sequencing capabilities can be very expensive in terms of equipment, time and manpower. A DNA sequencing facility would permit the acquisition of DNA sequence information with minimal expenditures. For established sequencers, there is an increasing desire to undertake large- scale sequencing projects involving hundreds of kilobases of DNA, however, using current manual sequencing techniques, projects of this magnitude are not feasible. These types of projects would be realistic through the use of a DNA sequencing facility. The DNA sequence of a gene is one of its most fundamental characteristics. Many key questions regarding the structure, expression and function of a gene can be addressed with a knowledge of the sequence. The availability of a DNA sequencing facility may permit investigators to explore avenues of research that might otherwise be impossible due to the lack of resources.