ANALYSIS OF POLYPEPTIDE CHANGES DURING CELLULAR DIFFERENTIATION

该项目的发起是为了定性和 从数量上讲，细胞总蛋白模式的变化 细胞衰老的定量二次荧光技术 空间聚丙烯酰胺凝胶电泳法。一个人类 已经开发出一种内皮细胞培养系统，它可以 一项研究细胞多肽表达变化的研究 衰老前期和衰老的人脐静脉内皮细胞 细胞(HUVEC)，并跟随细胞表型和 多肽在不同药物治疗后的表达 生物生长因子。二维聚丙烯酰胺凝胶 (35-S)-蛋氨酸标记鸡血球蛋白多肽的电泳法 青年早衰者(16人翻一番)和老年老年人 (55个种群倍增)人内皮细胞经 12-0-十四酰佛波醇-13-乙酸酯(TPA)，重组 γ-干扰素和肿瘤坏死因子 (肿瘤坏死因子)揭示了每个物种特有的多肽的表达 生物反应调节剂，也是衰老所特有的。 衰老前期人脐静脉内皮细胞表达13种不同的多肽 在衰老细胞和衰老的人内皮细胞中表达 细胞表达9种年轻细胞不表达的多肽。 此外，TPA和伽马-干扰素逆转了a的表达。 衰老前期和老年所特有的多肽的数量 衰老的细胞。

This project was initiated to analyze, both qualitatively and quantitatively, changes in total cellular protein patterns during cellular senescence using the technique of quantitative two- dimensional polyacrylamide gel electrophoresis. A human endothelial cell culture system has been developed which allows one to study changes in cellular polypeptide expression in presenescent and senescent human umbilical vein endothelial cells (HUVEC) and to follow changes in cellular phenotype and polypeptide expression following treatment with a variety of biological growth factors. Two-dimensional polyacrylamide gel electrophoresis of (35-S)-methionine-labelled polypeptides of young presenescent (16 population doublings) and old senescent (55 population doublings) human endothelial cells treated with 12-0-tetradecanoylphorbol-13-acetate (TPA), recombinant gamma-interferon (gamma-IFN), and tumor necrosis factor (TNF) revealed the expression of polypeptides unique to each biological response modifier and also unique to senescence. Presenescent HUVEC express 13 polypeptides that are not expressed in senescent cells and senescent human endothelial cells express 9 polypeptides that young cells do not express. Furthermore, TPA and gamma-IFN revert the expression of a number of polypeptides which are unique to presenescent and old senescent cells.