FLUOROIMMUNOASSAY APPARATUS

具有衰减时间常数的新型稀土螯合荧光探针 远长于典型的背景衰减时间常数。 有机材料提供了极大改善的潜力， 时间分辨荧光法的灵敏度。 然而，如果他们要 竞争性放射免疫测定方法的灵敏度要求变得 如此之大，荧光检测器过载恢复 主要问题。 过去关于这个问题的大量工作主要是 不适用于非常低的速度和水平的要求，这 形势 因此，正在作出初步努力， 确定最佳荧光检测装置和辅助装置 信号过载限制电路。 相关问题 荧光探针在极低的样品浓度将是 与CDC合作研究，CDC对此感兴趣， 作为艾滋病研究的另一种工具。 最后是评价 激发、光学滤波和数字信号的细节 将制定和优化处理。

New rare earth chelate fluorescent probes with decay time constants much longer than the background decay time-constant of typical. organic materials offer the potential of a great improvement in sensitivity of time-resolved fluorimetry. However, if they are to rival radioimmunoassay methods the sensitivity requirement becomes so great that overload recovery of the fluorescence detector is a major problem. Extensive past work on this problem is largely inapplicable to the very low speed and level requirements of this situation. Consequently initial effort is being applied to determining the optimum fluorescence detection device and ancillary signal overload limiting circuitry. Problems related to the fluorescent probe at extremely low sample concentrations will be studied in collaboration with CDC, which is interested in this approach as another tool for AIDS research. Finally an evaluation of the details of excitation, optical filtering, and digital signal processing will be worked out and optimized.