PURIFICATION OF MESSENGER RNAS ENCODING FOR NEUROTROPIC FACTORS IN RAT BRAIN

脑损伤与形态免疫学有关， 生物化学修饰，其中一些可能涉及 愈合过程 神经营养因子已经被证明 在大鼠脑损伤后。 该项目旨在分子 克隆编码这些损伤诱导的基因之一， 神经营养因子 cDNA文库已经从来自 损伤的大鼠大脑，这被证明是能够诱导 神经营养活性时注入非洲爪蟾卵母细胞。 通过差异菌落杂交筛选文库， 通过对融合蛋白神经营养活性的检测， 克隆导致了几个克隆的分离。 DNA 这些克隆的融合蛋白的测序和分析是 进行中。

Brain injury is associated with morphological immunological, and biochemical modifications, some of which may be involved with a healing process. Neurotrophic factors have been shown to appear in the rat brain after lesion. This project intends the molecular cloning of one of the genes encoding for these lesion-induced neurotrophic factors. A cDNA library has been constructed from a mRNA fraction from lesioned rat brain, which was shown to be capable of inducing neurotrophic activity when injected into Xenopus oocytes. Screening of the library by differential colony hybridization and by the test of the neurotrophic activity of the fusion protein the clones has lead to the isolation of several clones. DNA sequencing and analysis of the fusion-protein of these clones is in progress.